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Sialic Acid Analysis

Sialic Acids (Neuraminic Acids) are most often found at the terminal position of glycans, and therefore are a likely point of contact for glycan-ligand interactions.

Biopharmaceutical sialylation refers to the type and distribution of sialic acids in the glycans of therapeutic glycoproteins. Sialic acids can significantly influence the safety and efficacy profiles of these drugs. In particular, the in vivo half-life of some biopharmaceuticals correlates with the degree of oligosaccharide sialylation.>

Furthermore, the sialylation pattern can be a very useful measure of product consistency during manufacturing. Advances in our understanding of these issues have led the FDA, EMEA and other regulators to tighten their rules on glycoprofiling throughout the drug life cycle.

The two main types of neuraminic acid residues found in biopharmaceuticals produced in mammalian expression systems are N-acetyl-neuraminic acid (NeuAc) and N-glycolylneuraminic acid (NeuGc). These usually occur as terminal structures attached to galactose (Gal) residues at the non-reducing terminii of both N- and O-linked glycans.

Controlling the ratio of NeuAc to NeuGc is critical for biomanufacturers. The reason is that NeuAc is the desired, normal human-type sialylation, while NeuGc is found in non-human glycoproteins and is considered an undesired, aberrant form of sialylation for therapeutic glycoproteins.

Read more from Biopharmaceutical Sialylation

Sialic acid labeling kit HPLC chromatogram and DMB dye diagram

DMB Sialic Acid Labeling Kit

The LudgerTag DMB Sialic Acid Labeling Kit has been developed for the qualitative analysis of sialic acids
The kit contains reagents for the release of sialic acids from glycoproteins. Released sialic acids are conjugated with DMB dye by an amination-cyclisation reaction.

The kit contains reagents and materials for up to 22 samples including the sialic acid reference panel, and the N-acetyl neuraminic acid and N-glycolyl neuraminic acid standards.

View product documentation as PDF
View Certificate of Analysis as PDF

Part Number: LT-KDMB-A1

uR2 2.1x100mm UHPLC Column

The LudgerSep uR2 2.1x100mm UHPLC column has been developed for the analysis of sialic acids labeled with DMB ( 1,2-diamino-4,5 methylenedioxybenzene.2HCl) using the LudgerTag DMB Sialic Acid Labeling Kit.

Dimensions: 2.1mm x 100mm

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LS-UR2-2.1x100

$1,625.00

Sialic Acid Quantitation Kit

The SialiQuant Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA).

View product documentation as PDF

Part Number: KE-SIALIQ

Sialic Acid Aldolase

Converts sialic acid to N-acetylmannosamine & pyruvate. Sialic acids can then be quantitated by a coupled reaction with NADH and lactic dehydrogenase

View product documentation:
Specsheet    CoA   MSDS

Part Number: E-ALD01

CMP-Sialic Acid Synthetase

This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
The resulting molecule can then be covalently attached to an oligosaccharide by sialic acid transferase.

View product documentation as PDF

Part Number: E-CMP01

sialidase au image

Sialidase Au

recombinant from A. ureafaciens

View product documentation:
Specsheet    CoA    MSDS

Part Number - Amount of Enzyme
E-S001       - 60 µLs¹
E-S001-20   - 20 µLs¹
E-S001-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

$180.00$1,344.00
sialidase cp image

Sialidase Cp

Sialidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.

recombinant from C. perfringens

View product documentation:
Specsheet    CoA    MSDS

Part Number - Amount of Enzyme
E-S005       - 60 µLs¹
E-S005-20   - 20 µLs¹
E-S005-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

$180.00$1,344.00
sialidase sp image

Sialidase Sp

Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.

recombinant from Streptococcus pneumoniae in E. Coli

View product documentation:
Specsheet   CoA    MSDS

Part Number - Amount of Enzyme
E-S007       - 60 µLs¹
E-S007-20   - 20 µLs¹
E-S007-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

$180.00$1,344.00
NANA:a skeletal representation of N-acetyl neuraminic acid Neu5Ac

Quantitative NANA standard

The N-acetylneuraminic acid (NANA) standard is a quantitative standard of NIST-F and USP traceable Neu5Ac monosaccharide.

Amount: 100 nanomoles

View product documentation as PDF

Part Number: CM-NEU-AC-01

NGNA- a skeletal representation of NGNA

Quantitative NGNA Standard

The N-glycolyneuraminic acid (NGNA) standard is a quantitative standard of NIST-F and USP traceable Neu5Gc monosaccharide.

View product documentation as PDF

Part Number: CM-NEU-GC-01

Neu5,9Ac2 skeletal representation

Neu5,9Ac2

5-N-acetyl-9-O-acetyl neuraminic acid standard (Neu5,9Ac2)

View product documentation as PDF

~1 nmol (of which >750 pmol is Neu5,9Ac2)

Part Number: CM-NEU5,9AC2-01

sialic acid reference panel image

Sialic Acid Reference Panel

The Sialic Acid Reference panel is a mixture of different sialic acids forms (as detailed on chromatogram below) sourced from bovine submaxillary mucin.

It includes the following sialic acids: Neu5Ac; Neu5Gc; Neu5,7Ac2; Neu5,Gc9Ac; Neu5,9Ac2; Neu5,7,(8),9Ac3Gc.

Amount: approx. 1.25 nmols

View product documentation as PDF

Part Number: CM-SRP-01

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