Sialylated Core 1 Glycan

The sialylated core 1 glycan has one terminal NeuAc sialic acid linked α-3 to the galactose of the core 1 glycan. Other core 1 O-linked glycans we supply include disialylated core 1 glycan and disialylated core 2 glycan.

Form Dry. Dried by centrifugal evaporation from an aqueous solution.

Purity >90% pure as assessed by a combination of CE and HPLC.

HILIC HPLC profile of 2AB labelled C1S(3)1 glycan (see method conditions below) (Cat. #: CAB-C1S(3)1-01, Batch B413-02).

Storage Keep at -20°C both before and after dissolution. This product is stable for at least 5 years as supplied.

Shipping The product can be shipped at ambient when dry. After dissolution, ship on dry ice.

Handling Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimize exposure to elevated temperatures or extremes of pH.

Safety This product is non-hazardous and has been purified from synthetic sources certified to be free of all hazardous material including pathogenic biological agents.

Sialyated Core 1 Glycan References

Brockhausen I, Schachter H, Stanley P. O-GalNAc Glycans. In: Varki A, Cummings RD, Esko JD, et al., editors. Essentials of Glycobiology. 2nd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009. Chapter 9.

Mungul A, Cooper L, Brockhausen I, Ryder K, Mandel U, Clausen H, Rughetti A, Miles DW, Taylor-Papadimitriou J, Burchell JM. Sialylated core 1 based O-linked glycans enhance the growth rate of mammary carcinoma cells in MUC1 transgenic mice. Int J Oncol. 2004 Oct;25(4):937-43.

Burchell JM, Beatson R, Graham R, Taylor-Papadimitriou J, Tajadura-Ortega V. O-linked mucin-type glycosylation in breast cancer. Biochem Soc Trans. 2018 Aug 20;46(4):779-788. doi: 10.1042/BST20170483. Epub 2018 Jun 14.