Sialic Acid Quantitation Kit
The SialiQuant Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA).
Part Number: KE-SIALIQ
This kit includes reagents for 25 assays, each assay measuring from 1-200 nmoles of sialic acid.
In this method, N-Acetylneuraminic acid aldolase catalyzies the reversible reaction:
N-Acetylneuraminic acid N-Acetylmannosamine + Pyruvic acid
The pyruvic acid can be reduced to lactic acid by -NADH and lactic dehydrogenase:
Pyruvic Acid + -NADH Lactic Acid + -NAD
Under the proper conditions, the first forward reaction predominates, and when coupled with -NADH reduction of pyruvic acid, the reaction goes to completion. -NADH oxidation can be accurately measured spectrophotometrically.