Sialidase Testing Panel

The Sialidase Testing Panel contains a mixture of α2-3, α2-6, and α2-8 sialylated oligosaccharides labelled with either 2-AB or procainamide. They were developed as a process control for sialidase digestions. Applied alongside the samples, it demonstratesshows that the enzymes have the expected specificity.

Sialidase Testing Panel includes a mixture of five glycans:
1. 3′-Sialyl Lewis X [Neu5Ac-a2-3Gal-b1-4(Fuc-a1-3)GlcNAc] – contains α2-3 linked
sialic acid along with branched α1-3 fucose (introducing steric hindrance for some
enzymatic reactions)
2. Sialyllacto-N-tetraose a [Neu5Ac-α2-3Gal-β1-3GlcNAc-β1-3Gal-β1-4Glc] – linear
oligosaccharide containing α2-3 sialic acid attached to galactose
3. Disialyllactose [Neu5Ac-a2-8NeuAc-a2-3Gal-b1-4Glc] – linear oligosaccharide
containing α2-8 linked sialic acid
4. Sialyllacto-N-tetraose c [Neu5Ac-a2-6Gal-b1-4GlcNAc-b1-3Gal-b1-4Glc] – linear
oligosaccharide containing α2-6 sialic acid attached to galactose
5. Disialyllacto-N-tetraose [Neu5Ac-a2-3Gal-b1-3(Neu5Ac-a2-6)GlcNAc-b1-3Gal-b1-
4Glc] – branched sialic acid oligosaccharide

Form Dry. Dried by centrifugal evaporation from an aqueous solution

Size 100 pool

Molecular weight
3’-Sialyl Lewis X 940.3648
Sialyllacto-N-tetraose a 1118.4126
Disialyllactose 1044.3758
Sialyllacto-N-tetraose c 1118.4126
Disialyllacto-N-tetraose 1409.5080

Purity 89% pure as assessed by UHPLC

Storage Store at -20°C both before and after dissolution. This product is stable for at least 5 years as supplied

Shipping The product can be shipped at ambient when dry. After dissolution, ship on dry ice.

Handling Allow the unopened vial to reach ambient temperature. Centrifuge to ensure that most of the lyophilized material is at the bottom of the vial. Add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates.