Procainamide Labeling Kit
Procainamide labeling permits glycan identification by either mass spectrometry or UHPLC. Because of its improved ionisation efficiency compared to 2-AB labelling, it can permit identification of minor glycans (1% relative peak area) by ESI-MS.
Part Number Reductant
LT-KPROC-24 Sodium Cyanoborohydride
LT-KPROC-VP24 2-Picoline Borane
Procainamide labeling permits glycan identification by either mass spectrometry or (U)HPLC. Because of its improved ionisation efficiency compared to 2AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.
Ludger’s procainamide labeling system is suitable for N-glycans, O-glycans, GSL-glycans, heparin or any sugar with a reducing terminus and uses the same reductive amination labeling method that has been used for 2AB & 2AA.
The kit contains reagents for the conjugation of dye to the free reducing end of the glycan by a reductive amination reaction with essentially stoichiometric labeling. Any purified glycans with free reducing termini can be labeled. Up to 12 separate analytical samples per set of labeling reagents (24 samples in total for the kit). From 25 pmol up to 25 nmol glycans can be labeled per sample.
This LudgerTag kit, along with a post-labelling clean up plate for the samples (catalog# LC-PROC-96), has been validated in house at Ludger. Typical CVs for triplicate analyses were 5%.
Mass free dye: 235.33
Mass Added to Glycan 219.172
Fluorescence: λex = 310 nm, λem = 370 nm.
Structural Integrity No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.
Storage Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The reagents are stable for at least two years as supplied.
Shipping The product can be shipped at ambient temperature.
Kozak RP, Tortosa CB, Fernandes DL, Spencer DI Comparison of procainamide and 2-aminobenzamide labeling for profiling and identification of glycans by liquid chromatography with fluorescence detection coupled to electrospray ionization-mass spectrometry Anal Biochem. Oct 1;486:38-40 (2015)
Klapoetke S, Zhang J, Becht S, Gu X, Ding X, The evaluation of a novel approach for the profiling and identification of N-linked glycan with a procainamide tag by HPLC with fluorescent and mass spectrometric detection, J. Pharm. Biomed. Anal. 53 (2010) 315–324.
Lei Zhang, Shen Luo, and Baolin Zhang Glycan analysis of therapeutic glycoproteins MABS 2016, VOL. 8, NO. 2, 205–215
Crescioli S, Chiaruttini G, Mele S, Ilieva KM, Pellizzari G, Spencer DIR, Gardner RA, Lacy KE, Spicer JF, Tutt ANJ, Wagner GK, Karagiannis SN Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncology Journal of Allergy and Clinical Immunology Jan 31. pii: S0091-6749(18)30081-2 (2018)
Thomson RI, Gardner RA, Strohfeldt K, Fernandes DL, Stafford GP, Spencer DIR, Osborn HMI Analysis of Three Epoetin Alpha Products by LC and LC-MS Indicates Differences in Glycosylation Critical Quality Attributes, Including Sialic Acid Analytical Chemistry Content Jun 20;89(12):6455-6462 (2017)
2-Picoline Borane, Sodium Cyanoborohydride