Procainamide Labeling Kit

Procainamide labeling permits glycan identification by either mass spectrometry or UHPLC. Because of its improved ionisation efficiency compared to 2-AB labelling, it can permit identification of minor glycans (1% relative peak area) by ESI-MS.

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Part Number           Reductant
LT-KPROC-24        Sodium Cyanoborohydride
LT-KPROC-96        Sodium Cyanoborohydride
LT-KPROC-VP24   2-Picoline Borane

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Product Description

Ludger’s procainamide labeling kit is suitable for N-glycans, O-glycans, GSL-glycans, heparin or any sugar with a reducing terminus. It uses the same reductive amination labeling method that has been used for 2-AB and 2-AA. Procainamide labeling is suitable 25 pmol – 25 nmol samples.

The kit contains reagents for the conjugation of dye to the free reducing end of the glycan by a reductive amination reaction with essentially stoichiometric labeling. Any purified glycans with free reducing termini can be labeled.

Procainamide – 4-amino-N-(2-diethylaminoethyl) benzamide

procainamide molecular structure image

 

Procainamide labeling permits glycan identification by either mass spectrometry or (U)HPLC. Because of its improved ionisation efficiency compared to 2AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.

Procainamide LC-MS analysis of human IgG

Procainamide LC-MS analysis of human IgG

 

LC-MS Analysis of Complex Biological Samples-  Tsetse fly saliva

(U)HPLC profile of procainamide labelled N-glycans

Procainamide References

Kozak RP, Tortosa CB, Fernandes DL, Spencer DI Comparison of procainamide and 2-aminobenzamide labeling for profiling and identification of glycans by liquid chromatography with fluorescence detection coupled to electrospray ionization-mass spectrometry Anal Biochem. Oct 1;486:38-40 (2015)

Klapoetke S, Zhang J, Becht S, Gu X, Ding X, The evaluation of a novel approach for the profiling and identification of N-linked glycan with a procainamide tag by HPLC with fluorescent and mass spectrometric detection, J. Pharm. Biomed. Anal. 53 (2010) 315–324.

Lei Zhang, Shen Luo, and Baolin Zhang Glycan analysis of therapeutic glycoproteins MABS 2016, VOL. 8, NO. 2, 205–215

Crescioli S, Chiaruttini G, Mele S, Ilieva KM, Pellizzari G, Spencer DIR, Gardner RA, Lacy KE, Spicer JF, Tutt ANJ, Wagner GK, Karagiannis SN Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncology Journal of Allergy and Clinical Immunology Jan 31. pii: S0091-6749(18)30081-2 (2018)

Thomson RI, Gardner RA, Strohfeldt K, Fernandes DL, Stafford GP, Spencer DIR, Osborn HMI Analysis of Three Epoetin Alpha Products by LC and LC-MS Indicates Differences in Glycosylation Critical Quality Attributes, Including Sialic Acid Analytical Chemistry Content Jun 20;89(12):6455-6462 (2017)