This kit is used for the permethylation of glycans. Permethylation stabilizes sialic acids for MALDI mass spectrometry analysis, and aids linkage analysis studies (MALDI-MS, ESI-MS).
– Improves and enhances ionization efficiency of glycans on mass spec
– Increased glycan hydrophobicity enables LC-MS analysis
– Relative and absolute quantitation can be performed
– Stabilizes the sialic acid moieties
– Easier determination of branching and glycosidic linkage positions
– Enables detection of both neutral and acidic glycans in positive ion mode
Part Number Reductant.
LT-PERMET-VP96 2-Picoline Borane – 96 samples
Out of stock
|96 well Permethylation plate||1 Plate|
|Dimethyl sulfoxide (DMSO)||40.0 ml|
|Dichloromethane (DCM)||60.0 ml|
|96 well Balance Plate||1 Plate|
|96 well Plate Lid||1 Lid / Mat|
The permethylation plate (LC-PERMET-96) is scalable and can be used for any number of samples between 1 and 96. Store the unused kit reagents and the unused plate wells in the recommended storage conditions to enable their use for further sample preparation at a later date.
Note: Due to shipping restrictions of methyl iodide/iodomethane, we are unable to provide this component with the kit. We recommend purchasing iodomethane with purity ≥99.0% (GC) from your local chemical provider. Iodomethane Synonym: methyl iodide CAS Number: 74-88-4
|a. Preparation of glycans||As required|
|b. Addition of DMSO and sample to plate||15 minutes|
|c. Incubation||15 minutes|
|d. Addition of MeI to permethylation plate||15 minutes|
|e. Incubation||60 minutes|
|a. Extraction of samples||3-4 hours|
|b. Drying permethylated samples||10-30 minutes|
|c. Addition of 70% MeOH for storage||10-20 minutes|
A. Dell; A. J. Reaseon; Kay-Hooi Khoo; M. Panico; A. McDowell and H. R. Morris Mass Spectrometry of Carbohydrate containing Biopolymer Methods in Enzymology 230: 108-132 (1994)
I.Ciucanu; C. E. Costello Elimination of Oxidative Degradation during the per-O-Methylation of Carbohydrates J. Am. Chem. Soc. 125: 16213-16219 (2003)
I.Ciucanu Per-O-Methylation reaction of structural analysis of carbohydrates by mass spectrometry Analytica Chimica Acta 576: 147-155 (2006)
I.Ciucanu; R. Caprita Per-O-Methylation of neutral carbohydrates directly from aqueous samples for gas chromatography and mass spectrometry analysis Analytica Chimica Acta 585: 81-85 (2007)
Shubhakar, A., Kozak, R.P., Reiding, K.R., Royle, L., Spencer, D.I.R., Fernandes, D.L., Wuhrer, M. Automated High-Throughput Permethylation for Glycosylation Analysis of Biologics using MALDI- TOF-MS Analytical Chemistry. acs.analchem. 6b01639 (2016).