Permethylation Kit
This kit is used for the permethylation of glycans. Permethylation stabilizes sialic acids for MALDI mass spectrometry analysis, and aids linkage analysis studies (MALDI-MS, ESI-MS).
– Improves and enhances ionization efficiency of glycans on mass spec
– Increased glycan hydrophobicity enables LC-MS analysis
– Relative and absolute quantitation can be performed
– Stabilizes the sialic acid moieties
– Easier determination of branching and glycosidic linkage positions
– Enables detection of both neutral and acidic glycans in positive ion mode
Photo Guide of the Procedure
View product documentation as PDF
View Certificate of Analysis as PDF
Part Number Reductant
LT-PERMET-VP24 2-Picoline Borane
Kit Contents
| 96 well Permethylation plate | 1 Plate |
| Dimethyl sulfoxide (DMSO) | 40.0 ml |
| Dichloromethane (DCM) | 60.0 ml |
| 96 well Balance Plate | 1 Plate |
| 96 well Plate Lid | 1 Lid / Mat |
The permethylation plate (LC-PERMET-96) is scalable and can be used for any number of samples between 1 and 96. Store the unused kit reagents and the unused plate wells in the recommended storage conditions to enable their use for further sample preparation at a later date.
Note: Due to shipping restrictions of methyl iodide/iodomethane, we are unable to provide this component with the kit. We recommend purchasing iodomethane with purity ≥99.0% (GC) from your local chemical provider. Iodomethane Synonym: methyl iodide CAS Number: 74-88-4
| a. Preparation of glycans | As required |
| b. Addition of DMSO and sample to plate | 15 minutes |
| c. Incubation | 15 minutes |
| d. Addition of MeI to permethylation plate | 15 minutes |
| e. Incubation | 60 minutes |
| a. Extraction of samples | 3-4 hours |
| b. Drying permethylated samples | 10-30 minutes |
| c. Addition of 70% MeOH for storage | 10-20 minutes |
Permethylation References
A. Dell; A. J. Reaseon; Kay-Hooi Khoo; M. Panico; A. McDowell and H. R. Morris Mass Spectrometry of Carbohydrate containing Biopolymer Methods in Enzymology 230: 108-132 (1994)
I.Ciucanu; C. E. Costello Elimination of Oxidative Degradation during the per-O-Methylation of Carbohydrates J. Am. Chem. Soc. 125: 16213-16219 (2003)
I.Ciucanu Per-O-Methylation reaction of structural analysis of carbohydrates by mass spectrometry Analytica Chimica Acta 576: 147-155 (2006)
I.Ciucanu; R. Caprita Per-O-Methylation of neutral carbohydrates directly from aqueous samples for gas chromatography and mass spectrometry analysis Analytica Chimica Acta 585: 81-85 (2007)
Shubhakar, A., Kozak, R.P., Reiding, K.R., Royle, L., Spencer, D.I.R., Fernandes, D.L., Wuhrer, M. Automated High-Throughput Permethylation for Glycosylation Analysis of Biologics using MALDI- TOF-MS Analytical Chemistry. acs.analchem. 6b01639 (2016).
