Permethylation Kit
This kit is used for the permethylation of glycans. Permethylation stabilizes sialic acids for MALDI mass spectrometry analysis, and aids linkage analysis studies (MALDI-MS, ESI-MS).
– Improves and enhances ionization efficiency of glycans on mass spec
– Increased glycan hydrophobicity enables LC-MS analysis
– Relative and absolute quantitation can be performed
– Stabilizes the sialic acid moieties
– Easier determination of branching and glycosidic linkage positions
– Enables detection of both neutral and acidic glycans in positive ion mode
Photo Guide of the Procedure
View Product Documentation/SDS as PDF
View Certificate of Analysis as PDF
Part Number Reductant
LT-PERMET-VP24 2-Picoline Borane
$1,447
Product Description
Kit Contents
| 96 well Permethylation plate | 1 Plate |
| Dimethyl sulfoxide (DMSO) | 40.0 ml |
| Dichloromethane (DCM) | 60.0 ml |
| 96 well Balance Plate | 1 Plate |
| 96 well Plate Lid | 1 Lid / Mat |
The permethylation plate (LC-PERMET-96) is scalable and can be used for any number of samples between 1 and 96. Store the unused kit reagents and the unused plate wells in the recommended storage conditions to enable their use for further sample preparation at a later date.
Note: Due to shipping restrictions of methyl iodide/iodomethane, we are unable to provide this component with the kit. We recommend purchasing iodomethane with purity ≥99.0% (GC) from your local chemical provider. Iodomethane Synonym: methyl iodide CAS Number: 74-88-4
| a. Preparation of glycans | As required |
| b. Addition of DMSO and sample to plate | 15 minutes |
| c. Incubation | 15 minutes |
| d. Addition of MeI to permethylation plate | 15 minutes |
| e. Incubation | 60 minutes |
| a. Extraction of samples | 3-4 hours |
| b. Drying permethylated samples | 10-30 minutes |
| c. Addition of 70% MeOH for storage | 10-20 minutes |
Permethylation References
A. Dell; A. J. Reaseon; Kay-Hooi Khoo; M. Panico; A. McDowell and H. R. Morris Mass Spectrometry of Carbohydrate containing Biopolymer Methods in Enzymology 230: 108-132 (1994)
I.Ciucanu; C. E. Costello Elimination of Oxidative Degradation during the per-O-Methylation of Carbohydrates J. Am. Chem. Soc. 125: 16213-16219 (2003)
I.Ciucanu Per-O-Methylation reaction of structural analysis of carbohydrates by mass spectrometry Analytica Chimica Acta 576: 147-155 (2006)
I.Ciucanu; R. Caprita Per-O-Methylation of neutral carbohydrates directly from aqueous samples for gas chromatography and mass spectrometry analysis Analytica Chimica Acta 585: 81-85 (2007)
Shubhakar, A., Kozak, R.P., Reiding, K.R., Royle, L., Spencer, D.I.R., Fernandes, D.L., Wuhrer, M. Automated High-Throughput Permethylation for Glycosylation Analysis of Biologics using MALDI- TOF-MS Analytical Chemistry. acs.analchem. 6b01639 (2016).
