PBM Glycosidase Removal
The LudgerClean PBM Glycosidase removal plate was developed for the capture of endo- and exoglycosidases after deglycosylation to prevent contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove glycosidases or other proteins before mass spectrometry analysis of glycans.
Part Number: LC-PBM-96
The PBM Glycosidase Removal Plate is a 96 well membrane-bottom plate containing a specialized protein binding membrane with a nominal pore size of 0.45 µm. This product is designed for use with both the Velocity Vacuum Manifold System that can be purchased from QA-Bio or with other popular vacuum manifold systems. Glycans pass through the membrane whilst proteins are bound to the membrane allowing separation of these two components.
Post-exoglycosidase Cleanup For removal of exoglycosidase enzymes after glycan sequencing. This will prevent contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove exoglycosidases or other proteins before mass spectrometry analysis of glycans.
Post-endoglycosidase Cleanup For removal of PNGase F by the and other protein and peptide material following the acidification of N-glycans following their release from glycoproteins. Using this protein binding membrane will provide cleaner samples and increase the signal for the subsequent fluorescent labelling of the N-glycans. The plate can also be used to remove PNGase F and other protein and peptide material before permethylation and/or mass spectrometry analysis of N-glycans.
Prior to cleaning up N-glycans following PNGase F digestion we recommend hydrolysing the N-glycans with 1% formic acid. The addition of 1 % formic acid solution to released N-glycan samples aids in the hydrolysis of the glycosylamine form of the N-glycans following PNGase F release. Hydrolysing the glycosylamine promotes the formation of a reducing end which enables the glycans to be fluorescently labelled.
Number of Samples Sufficient for up to 96 samples.
Amount of Sample Up to 350 µL per well
Suitable Samples Unlabelled glycans from glycoproteins, fluorophore labelled glycans e.g. 2-AB or 2-AA from glycoproteins, released and acidified N-glycans from glycoproteins.
Storage Store the PBM Glycosidase Removal Plate at room temperature. Protect from sources of heat, light, and moisture. When stored correctly, the reagents should be stable for 36 months from date of purchase.
Shipping The product should be shipped at ambient temperature.