ManMix Oligomannose Reference Panel

The ManMix oligomannose reference panel is a mixture of high mannose N-linked glycans including Man-5, Man-6, Man-7, Man-8, and Man-9.

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CLIBN-MANMIX-10U 10 µg
CLIBN-MANMIX-20U 20 µg

Product Description

The ManMix Oligomannose Reference Panel is a mixture of high mannose glycan standards. The panel includes Man-5, Man-6, Man-7, Man-8, and Man-9 with over 90% purity as assessed by HPLC.

Our oligomannose reference panel is a test standard used both in FDA- and EMA-approved assays worldwide. This library is typically used as a reference standard for high mannose glycans  monitored in the manufacturing of biotherapeutics. It can also be implemented as a process control to assess the performance of a glycan labelling protocol and/or a system suitability standard.

This oligomannose reference panel combines six oligomannose N-glycan oligosaccharides extracted from porcine thyroglobulin and qualified by MS, HPLC and NMR analysis to meet the high purity and quality standards required by advanced analytical techniques.

 

Mannose Reference Panel Table 1 Image
Table 1. Glycan names and structures of each peak from the chromatogram.

 

Our oligomannose reference panel standards are supported by complete documentation. The certificate of analysis contains the results from the testing used to characterize the material across a complete range of quality characteristics. These standards are well-characterised with documented purity. Each oligomannose reference panel vial contains approximately 10µg of oligomannose N-glycans.

 

Chromatogram of procainamide-labelled ManMix mannose reference panel image

 

Molecular Weight:
Man-5 1235 dalt0ns
Man-6 1398 daltons
Man-7 1560 daltons
Man-8 1722 daltons
Man-9 1884 daltons

Purity: > 90% pure as assessed by HPLC

Form: Dry. Dried by centrifugal evaporation from an aqueous solution.

Oligomannose Reference Panel Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied. View Certificate of Stabilty

Shipping: The product can be shipped at ambient when dry. After dissolution, ship on dry ice.

Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.

Safety: The oligomannose reference panel is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.