The Neu5,9Ac2 standard standard is a mixture of various sialic acids. The main sialic acid species present, 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2), makes up 77% of the mixture. This product is only for use as a chromatography standard and should be not used as a quantitative standard.
Ludger Guides to Sialic Acid Analysis:
Sialylation Guide I: Neu5Ac and Neu5Gc Quantitation
Sialylation Guide II: Highly Sialylated Glycoproteins
Application Note: Quantitative Sialic Acid Analysis
LudgerSep-R1 HPLC profile of 1,2-diamino-4,5-methylenedioxybenzene.2HCl (DMB) labeled Neu5,9Ac2 standard
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Amount Supplied: ~1 nmol (of which 770 pmol is Neu5,9Ac2)
Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.
View Certificate of Stabilty
Shipping: The NGNA standard can be shipped at ambient when dry. After dissolution, ship on dry ice.
Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.
Safety: This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.
Download Non-hazardous MSDS
Fernandes, D. Biopharmaceutical Sialylation. European Biopharmaceutical Review. Spring 2006. pp 100 -104
Bhide GP, Colley KJ. Sialylation of N-glycans: mechanism, cellular compartmentalization and function. Histochem Cell Biol. 2017 Feb;147(2):149-174. doi: 10.1007/s00418-016-1520-x. Epub 2016 Dec 14.
Anumula KR. Rapid quantitative determination of sialic acids in glycoproteins by high-performance liquid chromatography with a sensitive fluorescence detection. Analytical Biochemistry 230: pp24-30, 1995,