Widely used for profiling of 2-AB and 2-AA labeled glycans as part of lot release QC for therapeutic glycoproteins.
The column contains particles coated in a robust amide polymer. These bind to glycans under conditions of high organic solvent. Glycans are separated and eluted from the column using solvent gradients with decreasing organic solvent content.
N1 4.6x250mm HPLC profile of 2-AB labeled fetuin glycans released by PNGase F