LS-C-BUFFX4

Application For preparation of LS-C solvent (500 mM ammonium formate buffer pH 9, 20 % acetonitrile v/v) used in WAX (weak anion exchange) HPLC analysis of LudgerTag™ fluorophore and UV-chromophore labeled glycans.

Description 50mL of x4 LS-C buffer concentrate (2.0M ammonium formate buffer/solution pH9.0) in a square HDPE bottle with leak-proof cap.

Usage Dilute the whole contents of the bottle (50mL) with 150 mL HPLC grade water, then add 50 mL acetonitrile to make LS-C solvent (500 mM ammonium formate H 9, 20% acetonitrile v/v). The 50 mL of x4 buffer will make 250 mL of LS-C solvent.

Use the LS-C solvent for glycan analysis with any of the following HPLC columns:
LudgerSep C3 column

Other weak anion exchange HPLC columns for glycan analysis

Storage Store unopened bottle below 25 oC. As with any HPLC solvent we recommend preparation of immediately before use. Take care that the undiluted/diluted solvent is not exposed to excessive heat or sunlight as it contains volatile components. Stability times of the prepared solvent will vary according to your laboratory conditions. Long term storage of the prepared solvent, ie longer than 1 month, at room temperature, may result in evaporation of some of the volatile components in the solvent leading to a change in retention times.

Shipping The product should be shipped at ambient temperature.

Handling: Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: Please read the Material Safety Data Sheets (SDS’s) for all chemicals used.All processes involving hazardous reagents should be performed using appropriate personal safety protection – eyeglasses, chemically resistant gloves (e.g. nitrile), etc. – and where appropriate in a laboratory fume cupboard.