IgG Glycoprotein Standard

This IgG glycoprotein standard was developed for use to be used as a positive control during glycan release and labeling.

Source This product is purified from human blood. Human IgG glycoprotein standard contains a mixture of gamma-type antibodies.

Molecular Weight ~150kDa (protein weight only)

Amount In comparison to BSA standard, determined by BCA assay. Value rounded to nearest μg:
GCP-IGG-50U     50 μg protein
GCP-IGG-100U 100 μg protein

Glycans The majority of glycans present on human antibodies are N-link bi-antennary types . These glycans exist in both fucosylated and non-fucosylated forms, with and without bisecting GlcNAcs, and terminate either with 0, 1 or 2 galactoses (the G0, G1 and G2 glycan forms). Sialic acid terminating glycans are also present on native human IgGs.

LudgerSepN2 (Cat. #: LS-N2-4.6×150) HPLC profile of 2-AB labelled IgG N-glycans, released by PNGase F from GCP-IGG-50U batch B13T-06. See product CoA for peak assignment.

Exoglycosidase analysis of Human IgG N-glycans from a similar batch of GCP-IGG. LudgerSep N2 chromatograms are shown.

Form Dry. Lyophilised powder.

Storage Keep at -20°C both before and after dissolving. This product is stable for at least 5 years as supplied.

Shipping The product is shipped at ambient temperature.

Handling Once dissolved avoid repeated thawing and refreezing, storage over 3 hours at room temperature or above, and exposure to light and long term exposure to acid as these will cause glycan desialylation.

Safety This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.