Glycopeptide Enrichment Cartridges

The LudgerClean A Cartridges have been developed for glycopeptide enrichment from a peptide/glycopeptide mixture after protease digestion.

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View Certificate of Analysis as PDF

Contains 24 cartridges

Part Number: LC-A-24

$1,350

Product Description

Glycopeptide enrichment following protease digestion can be achieved using LudgerClean A Cartridges, enhancing your UHPLC and/or MS signals. Glycopeptides signals are attenuated in a complex mixture, as they are suppressed by non-glycosylated peptides. Enrichment can be performed before or after a labeling reaction.

Glycopeptide Enrichment Workflow:

Glycopeptide Enrichment Workflow

 

LudgerClean A cartridges were developed to be used with any 96 well micro-titre plate with a vacuum manifold (E.g. Ludger-Velocity Vacuum Manifold System – LC-VAC-MANIFOLD-KIT) or a liquid handling robot.

 

UHPLC profile of labeled glycopeptides after enrichment

Glycopeptide Enrichment-tag
Vtag IgG1 labeled glycopeptide UHPLC profile, LC-A enrichment was performed after labeling

 

glycopeptide enrichment - AQC labeled
AQC IgG1 labeled glycopeptide UHPLC profile, LC-A enrichment was perform

 

MS analysis of labeled glycopeptides after enrichment

Vtag IgG1 labeled glycopeptide MALDI-MS profile, LC-A enrichment was performed after labeling

 

glycopeptide MALDI-MS AQC labeled
AQC IgG1 labeled glycopeptide MALDI-MS profile, LC-A enrichment was performed after labeling

 

AQC IgG1 labeled glycopeptide Base peak chromatogram in positive mode ESI-MS profile, LC-A enrichment was performed after labeling