Glycopeptide Enrichment Cartridges

Glycopeptide enrichment following protease digestion can be achieved using LudgerClean A Cartridges, enhancing your UHPLC and/or MS signals. Glycopeptides signals are attenuated in a complex mixture, as they are suppressed by non-glycosylated peptides. Enrichment can be performed before or after a labeling reaction.

Glycopeptide Enrichment Workflow:

LudgerClean A cartridges were developed to be used with any 96 well micro-titre plate with a vacuum manifold (E.g. Ludger-Velocity Vacuum Manifold System – LC-VAC-MANIFOLD-KIT) or a liquid handling robot.

UHPLC profile of labeled glycopeptides after enrichment

MS analysis of labeled glycopeptides after enrichment