Enzymatic CarboRelease Kit
Kit includes the enzymes and buffers required to remove all N-linked oligosaccharides and many O-linked sugars from 2 mg of glycoprotein, via twenty 100 microgram samples.
The enzymes are packaged separately in individual 20 microlitre vials. This gives researchers the flexibility to characterize the glycans attached to their glycoprotein more fully than with our mixture of enzymes, KE-DGMX.
Part Number: KE-DG01
Appropriate use of individual glycosidases allow the determination of sialyation (use of Sialidase only) and the specific presence of N-linked glycans (using PNGase F only) and O-linked glycans (using Sialidase, Beta-Galactosidase, O-Glycosidase, and Glucosaminidase).
Other Supplied Reagents:
2. Add 2.5 µl denaturation solution. Mix gently and place in boiling water bath for 5 minutes. Chill on ice.
3. Add 2.5 µl of Triton-X.
4. Add 1 µl each of PNGase F, Sialidase, Beta-Galactosidase, Glucosaminidase, and O-Glycosidase. Incubate for 3 hours at 37˚C.
Note: Denaturation increases the rate of enzyme digestion up to 10 fold. If denaturation is not desired omit step 2-3, add with 5 µl of distilled water and increase incubation time to 24 hours.