Part number E-EF03
Endo F3, Endoglycosidase F3, endo-beta-N-acetylglucosaminidase F
Source recombinant Elizabethkingia meningosepticum (was Chryseobacterium meningosepticum)
Cleaves all asparagine-linked triantennary or fucosylated biantennary oligosaccharides, as well as triamannosyl chitobiose core structures. Alpha 1-3 fucosylation will inhibit enzymatic activity. (Note:
The recombinant version is not glycosylated, which may result in properties differing from the native protein.)
60 µl aliquot of enzyme (0.33 U) in 20 mM Tris-HCl, pH 7.5
5x Reaction Buffer – 250 mM sodium acetate, pH 4.5
Specific Activity >25 U/mg
Activity 5 U/ml
Molecular weight 30,000 daltons
1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.
2. Add 10 µl 5x Reaction Buffer 4.5
4. Add 2.0 µl of Endo F3. Incubate 1 hour at 37˚C.
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of porcine fibrinogen in 1 minute at 37˚C, pH 4.5. Cleavage is monitored by SDS-PAGE (cleaved fibrinogen migrates faster).
Storage Store enzyme at 4˚C.