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Endo F1

Selective release of high mannose and some hybrid type N-glycans from peptides and proteins

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Specsheet CoA MSDS

Part Number: E-EF01


Part number E-EF01

Endo F1, Endoglycosidase F1, endo-beta-N-acetylglucosaminidase F

Source recombinant Elizabethkingia miricola (was Chryseobacterium meningosepticum) in E. Coli


Specifictity Cleaves all asparagine-linked hybrid or high mannose oligosaccharides

Contents 60 µl aliquot of enzyme (1 U) in 20 mM Tris-HCl, pH 7.5

5x Reaction Buffer – 250 mM sodium phosphate, pH 5.5

Specific Activity >16 U/mg

Activity 17 U/ml

Molecular weight 32,000 daltons

Suggested usage
1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.
2. Add 10 µl 5x Reaction Buffer 5.5
3. Add 2.0 µl of Endo F1. Incubate 1 hour at 37ˆC.

Specific Activity
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured Ribonuclease B (RNase B) in 1 minute at 37˚C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved RNase B migrates faster).

Storage Store enzyme at 4˚C.

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