Endo F Multi-Kit
The Endo F family of enzymes cleave asparagine-linked oligosaccharides between the two GlcNAc residues in the core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine.
The remaining monosacharide imparts a charge, thereby increasing the solubility of the protein. In contrast, PNGase F removes the oligosaccharide intact.
Part Number: KE-EFX3
– Deglycosylation of native proteins resistant to PNGase F cleavage
– Determination of glycan type (high mannose, biantennary, tri/tetrantennary)
– Deglycosylating proteins which normally precipitate when deglycosylating
– X-Ray Crystallography
20 µl of Endo F1
20 µl of Endo F2
20 µl of Endo F3