The disialylated core 2 glycan consists of a galactose molecule linked β-3 to GalNAc similar to the core 1 glycan. NeuAc sialic acid is linked alpha-3 to the galactose while the GalNac is attached to GlcNAc-Gal-NeuAc. This glycan is labeled with the fluorescent 2-AB molecule.
Form Dry. Dried by centrifugal evaporation from an aqueous solution.
Purity >90% pure as assessed by a combination of CE and HPLC.
HILIC HPLC profile of 2AB labelled C2S(3,3)2-01 glycan (see method conditions below) (Cat. #: CAB-C2S(3,3)2-01, Batch B67L-01).
Storage Keep at -20°C both before and after dissolution. This product is stable for at least 5 years as supplied.
Shipping The product can be shipped at ambient when dry. After dissolution, ship on dry ice.
Handling Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimize exposure to elevated temperatures or extremes of pH.
Safety This product is non-hazardous and has been purified from synthetic sources certified to be free of all hazardous material including pathogenic biological agents.
Core 2 Glycan References
Brockhausen I, Schachter H, Stanley P. O-GalNAc Glycans. In: Varki A, Cummings RD, Esko JD, et al., editors. Essentials of Glycobiology. 2nd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009. Chapter 9.