LudgerZyme Ceramide Glycanase
Ceramide glycanase can be used to deglycosylate a variety of glycosphingolipids by cleaving the β-glycosyl linkage.
Part number: LZ-CER-HM-KIT
Part number LZ-CER-HM-KIT
Source Hirudo medicinalis
Ceramide glycanase is an enzyme used to release glycans from glycosphingolipids (GSLs) to enable their characterisation. It cleaves glycans including GM1, GM2 and GM3 by cleaving the β-glycosyl linkage. Glycans can then be labelled using LudgerTag labelling technology.
Number of Samples/ Sufficient for 25 samples
Amount of Samples Up to 1 nmol of glycolipid
Suitable of Samples Isolated glycolipids containing a ceramide backbone.
1. Pipette 5 μl of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 μl of approximately 1nmol of the glycolipid sample to be tested.
2. For the positive control reaction, add 5 μl of GLIP-GM1-01 substrate to 5 μl of LZ-CER-BUFFX4 into an Eppendorf tube
3. Add 2 μl of LZ-CER-HM-10 (the enzyme) to the reaction mixture and mix gently by tapping the Eppendorf tube with your finger. Make total reaction mix up to 20 μl with water.
4. Incubate your samples in a water bath, oven or any other constant heating source at 37°C for 24 hrs.
The samples are now ready for procainamide, 2-AB or 2-AA labeling and subsequent analysis by HPLC. Samples can be stored frozen at this point.
Long term storage – over 14 days – store at – 20°C.
Protect from sources of heat and light. Avoid multiple freeze thaw cycles.
Short term storage – up to 14 days – store at 4 °C.
Protect from sources of heat and light.