BioQuant Mannose 6-Phosphate

The BioQuant Mannose 6-Phosphate standard is a quantified glycan standard. The standard has been accurately dispensed from a bulk solution, the concentration of which has been determined by quantitative NMR. 

Product documents:
Certificate of Analysis Search
SDS

Glycan Purity determined as > 90% by UHPLC

Amount supplied: 10 mol

Part number: CM-MAN6P-10

US$133

Product Description

Mannose 6-phosphate (Man6P) is a phosphorylated form of the monosaccharide mannose. Mannose 6-phosphate is a critical molecule in intracellular trafficking, specifically directing proteins to lysosomes for degradation.

Man6P, a terminal monosaccharide of asparagine-linked oligosaccharides, is a key targeting signal for acid hydrolase precursor proteins that are destined for transport to lysosomes and is present in therapeutic enzymes (enzyme replacement therapies) developed for the treatment of lysosomal storage diseases.

Lysosomal Targeting: M6P serves as a molecular tag for specific proteins that are destined for transport to lysosomes. Proteins targeted for degradation in lysosomes carry M6P residues on their surface.
Glycosylation Modification: M6P is added to specific sugar residues on proteins during post-translational modification within the Golgi apparatus. This modification is essential for directing proteins toward lysosomes.
Mucolipidosis and I-Cell Disease: Genetic defects in the enzymes responsible for M6P addition can lead to lysosomal storage disorders, such as mucolipidosis and I-cell disease. These conditions are characterized by impaired targeting of lysosomal enzymes, resulting in their secretion outside the cell instead of being delivered to lysosomes.
Receptor-Mediated Uptake: Cells possess M6P receptors on their surfaces, which recognize and bind to proteins marked with M6P. This binding triggers cellular processes that facilitate the internalization of the M6P-tagged proteins into lysosomes.

Form: Dry. Dried by centrifugal evaporation from an aqueous solution.

Amount: 10 nmol

Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.

Shipping: Shipped at ambient when dry. After dissolution, ship on dry ice.

Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharides into the solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates—Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.

Safety: This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.