Chitotriose – BioQuant
The chitotriose quantatative standard is a linear tri-N-acetylglucosamine glycan.
Quantity determined by quantitative Nuclear Magnetic Resonance (qNMR).
Can be used as an internal or external standard, spiked directly into your glycan sample or run in parallel.
Amount: 5 ± 0.30 nmol by qNMR
Part Number: BQ-CHITOTRIOSE-01
The chitotriose quantatative standard is a linear tri-N-acetylglucosamine glycan, available as an unlabeled glycan or labeled with 2-AB and 2-AA. The amount of chitotriose to be dispensed per vial has been determined by quantitative nuclear magnetic resonance (qNMR) of the bulk chitotriose stock.
Accurate Quantity Determined by quantitative NMR <br> 5nmols of the lyophilised standard supplied in a 0.5mL vial.
Ready for LudgerTag Labeling The sugar has a free reducing end that readily accepts a fluorescent tag.
Regulatory Submissions Support system suitability and drugs regulatory submission by demonstrating consistent and reproducible glycosylation levels
Reliable Quantification Method Follows established glycan analysis techniques. Provides data comparable to gold-standard glycoprofiling methods based on Monosaccharide analysis
Quick and Easy Used in QC routine in-house to determine quantity of both bulk and dispensed glycans
Use as Internal or External Quantitative Standard The Chitotriose standard can be spiked directly into your glycan sample or run in parallel.
Integrates Easily With Fluorescent Labeling Workflows Adds into your existing labeling workflow, without requiring any extra steps.
Use as an Internal Standard Our unlabeled chitotriose standard can be used as an internal standard to quantify glycans in your sample. To do this, add a known amount of the unlabeled chitotriose to your unlabeled glycan sample. The mixture is then fluorescently labeled, cleaned up and run on HPLC/UHPLC. The unknown quantity of the glycan can be inferred by comparison of the chitotriose and glycan peak areas. The advantage of this method is that any sample loss occurring during the labelling and clean up stages will be the same for the sample and the chitotriose, thereby removing this as a source of error. The results obtained when running this method compare favourably with monosaccharide testing.
Use as External Standards The 2-AB and 2-AA labeled standards (Cat: # BQ-CAB-CHI-01 and Cat: # BQ-CAA-CHI-01 respectively) can be used as external standards to quantify glycans; these standards can be run directly on the HPLC/UHPLC. Again, the quantity of glycans in your sample is inferred by comparison of the peak areas for chitotriose (known amount) and your sample. This method is useful when quantification of already labeled glycans is required. They are also useful when quantification of several glycan samples is required in the same run, as the labeled chitotriose standard can be used as external standard for all the samples.
BioQuant Chitotriose Workflow
BioQuant Data Analysis