Alpha Mannosidase

Alpha Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.

View product documentation:
Specsheet
CofA

SDS: US   EU

Part Number – Amount of Enzyme
E-AM01       – 60 µLs¹
E-AM01-20   – 20 µLs¹
E-AM01-200 – 200 µls²
¹ includes buffer
² includes enzyme only

Product Description

alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, alpha mannosidase, exo-alpha-mannosidase

Alpha Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.

This enzyme is often used in conjunction with Core Alpha-(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate.

Source Jack Bean

EC 3.2.1.24

Contents
Alpha-(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5).

Included with 20 µL and 60 µL pack sizes:
200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0).

Specific Activity >3 U/mg
Activity >10 U/ml

Molecular weight two polypeptides of 64,000 and 44,000 daltons

Suggested usage
1. Add up to 1 nmol of oligosaccharide.
2. Add deionized water to 15 µl.
3. Add 4 µl 5x Reaction Buffer 5.0.
4. Add 1 µL of enzyme
5. Incubate ten minutes at 37˚C.

Specificty All Alpha-(1-2,3,6)-linked mannose

Specific Activity Assay
One unit of Alpha Mannosidase is defined as the amount of enzyme required to hydrolyze 1 µmole of p-nitrophenyl-alpha-p-mannoside to p-nitrophenol in 1 minute at pH 5.0 and 37˚C.

Storage Store enzyme at 4˚C.

Purity Each lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 μU/mL (IUB).

For the protease assay, 10 μg of denatured BSA is incubated for 24 hours with 2 μl of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.

Stability Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.

Alpha Mannosidase References

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Snaith, S.M., and G.A. Levvy. Purification and Properties of α-D- Mannosidase from Jack-Bean meal. Biochem. J. 110: 633-670 (1968)

Ganesh Kumar, B.S., Pohlrntz, G., Shculte, M., Mormann, M. Siva Kumar, N. Jack bean α-mannosidase: amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool. Glycobiology 24(3):252-61 (2014)