Alpha (1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures.
Part Number – Amount of Enzyme
E-F134 – 60 µLs¹
E-F134-20 – 20 µLs¹
E-F134-200 – 200 µls²
¹ includes buffer
² includes enzyme only
$198 – $1,377
alpha-L-fucoside fucohydrolase, alpha-L-fucosidase, alpha-(1-3,4) fucosidase
Alpha (1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.
For removing core fucose linked α-(1-6) to the core GlcNAc of a GlcNAc-GlcNAc disaccharide structure we recommend our Alpha-(1-6) Fucosidase (E-F006).
α(1-3, 4) Fucosidase is useful for:
nbsp;nbsp;Fucose linkage determination
nbsp;nbsp;Deglycosylating glycoproteins with Lewis structures
Source Isolated from Xanthamonas manihotis
Alpha-(1-3,4)-Fucosidase in 20 mM Tris-HCl, 25 mM NaCl,(pH 7.5).
Included with 20 µL and 60 µL pack sizes:
5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)
Specific Activity >2 U/mg
Activity 0.5 U/ml
Molecular weight 40,000 daltons
Formulation The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl pH 7.5.
1. Add up to 1 nmole of oligosaccharide to a tube.
2. Add de-ionized water to a total of 15 µl.
3. Add 4 µl of 5x Reaction Buffer 5.0.
4. Add 1 µl of Alpha-(1-3,4)-Fucosidase.
5. Incubate for 1 hour at 37˚C.
Specific Activity Assay One unit of Fucosidase activity is defined as the amount of enzyme required to cleave 1 µmole of fucose from Lewis X trisaccharide, 4-methylumbelliferyl glycoside in 1 minute at 37˚C and pH 5.0. Lewis X trisaccharide is Gal Beta-(1-4)[Fuc alpha-(1-3)]GlcNAc.
Storage Store enzyme at 4˚C.
Purity Each lot of α(1-3, 4) Fucosidase is tested for contaminating activities by incubating
the enzyme for 24 hours at 37°C with the appropriate substrates; the detection limit of this assay is 5 μU/mL (IUB). A passing lot will have no detectable activity.
For the protease assay, 10 μg of denatured BSA is incubated for 24 hours with 2 μL of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.
Stability Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.