Sialate O-acetylesterase kit

Acetyl esterase, sialate-O-acetylesterase

The LudgerZyme Acetyl Esterase Kit removes 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins. It is commonly used for the characterization of highly-sialylated biotherapeutics such as EPO, FSH and blood clotting factors.

Source recombinant from Tannerella forsythia in E. Coli.

Contents
Acetyl esterase – PBS pH7.5 buffer containing 10 mM Tris-HCl
Reaction Buffer – 500 mM sodium acetate pH5.5

Amount Supplied Sufficient for up to 50 samples.

Enzyme Commission Number EC 3.1.1.53

Number of Samples Sufficient for up to 50 samples.

Amount of Sample Up to 10 μg glycoprotein, up to 2.5 μg released glycans and up to 1 μg free sialic acid per digestion.

Suitable Samples Acetyl esterase (sialate-O-acetylesterase) can act upon complex glycoprotein samples, such as erythropoietin (EPO), bovine submaxillary mucin and oral epithelial cell-bound glycans, and on N- and O-glycans released from a glycoprotein. Either fluorescently labelled or unlabelled glycans are suitable. It can also be used on released sialic acids.

Unit Definition One unit (U) of acetyl esterase is defined as the amount of enzyme required to produce 300 μmole of 4-nitrophenol and acetate in 1 minute at 30°C in a buffer containing 50 mM Tris-HCl, 140 mM NaCl, pH 8.5, from 4-nitrophenyl acetate, a chromogenic esterase substrate.

Molecular Weight 76.3 kD

Storage Store at 4°C. Protect from sources of heat and light. When stored correctly, the enzyme should be stable for 24 months from date of purchase. Exposure to ambient temperatures (20 – 26°C) over 3 days does not result in a reduction of enzymatic activity.

Shipping The product should be shipped at 4°C.