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Sialic Acid Products

Sialic Acids are most often found at the terminal position of glycans, and therefore are a likely point of contact for glycan-ligand interactions.

Biopharmaceutical sialylation refers to the type and distribution of sialic acids in the glycans of therapeutic glycoproteins. Sialylation can significantly influence the safety and efficacy profiles of these drugs. In particular, the in vivo half-life of some biopharmaceuticals correlates with the degree of oligosaccharide sialylation.

Furthermore, the sialylation pattern can be a very useful measure of product consistency during manufacturing. Advances in our understanding of these issues have led the FDA, EMEA and other regulators to tighten their rules on glycoprofiling throughout the drug life cycle.

The two main types of sialyl residues found in biopharmaceuticals produced in mammalian expression systems are N-acetyl-neuraminic acid (NeuAc) and N-glycolylneuraminic acid (NeuGc). These usually occur as terminal structures attached to galactose (Gal) residues at the non-reducing terminii of both N- and O-linked glycans.

Controlling the ratio of NeuAc to NeuGc is critical for biomanufacturers. The reason is that NeuAc is the desired, normal human-type sialylation, while NeuGc is found in non-human glycoproteins and is considered an undesired, aberrant form of sialylation for therapeutic glycoproteins.

Read more from Biopharmaceutical Sialylation

DMB Sialic Acid Labeling Kit

The LudgerTag DMB Labeling Kit has been developed for the qualitative analysis of sialic acids

The kit contains two sets of the labeling reagents and a sialic acid reference panel (pictured):
- Sialic Acid Reference Panel
- DMB Dye (1,2-diamino-4,5-methylenedioxybenzene.2HCl)
- Acetic Acid
- Mercaptoethanol in Acetic acid
- Sodium Dithionite (Reductant)

Typically, up to 15 separate analytical samples can be labeled per set.

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KDMB-A1

uR2 2.1x100mm UHPLC Column

The LudgerSep uR2 2.1x100mm UHPLC column has been developed for the analysis of sialic acids labeled with DMB ( 1,2-diamino-4,5 methylenedioxybenzene.2HCl) using the LudgerTag DMB Sialic Acid Labeling Kit.

Dimensions: 2.1mm x 100mm

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LS-UR2-2.1x100


Sialic Acid Quantitation Kit

The SialiQuant Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA).

View product documentation as PDF

Part Number: KE-SIALIQ

Sialic Acid Aldolase

Converts sialic acid to N-acetylmannosamine & pyruvate. Sialic acids can then be quantitated by a coupled reaction with NADH and lactic dehydrogenase

View product documentation as PDF

Part Number: E-ALD01

CMP-Sialic Acid Synthetase

This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
The resulting molecule can then be covalently attached to an oligosaccharide by sialic acid transferase.

View product documentation as PDF

Part Number: E-CMP01

Sialidase Au

recombinant from A. ureafaciens

View product documentation:
Specsheet    CoA   MSDS

Part Number: E-S001

Sialidase Cp

recombinant from C. perfringens

View product documentation:
Specsheet    CoA   MSDS

Part Number: E-S005

Sialidase Sp

recombinant from Streptococcus pneumoniae in E. Coli</pView product documentation: Specsheet  CoA  MSDS Part Number: E-S007
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