Tools for Glycan Analysis
Sialic Acids (a family of derivatives of neuraminic acid) are usually found as terminal structures of both N-linked and O-linked glycans.
Being located at the terminal position of glycans, sialic acids are a likely point of contact for many glycoprotein interactions. They are also important for the stability and 3D conformation of glycoproteins and are involved in many biological interactions:
• Sialyation of IgG reduces ADCC and increases anti-inflammatory activity
• Sialic acids increase the serum half-life of glycoproteins by preventing uptake by the liver’s asialoglycoprotein receptor
• NGNA(Neu5Gc), a glycan not found in humans, can illicit an immune response and lead to increased neutralization of biopharmaceuticals.
• Cell line choice can greatly influence the type of neuraminic acids present on a biopharmaceutical, for example a large portion of the sialic acids on mouse IgG are often NGNA.
The Sialidase Testing Panel is a mixture of five different oligosaccharides containing NeuAc sialic acid linked in α2-3, α2-6, and α2-8 positions. The glycans are labeled with either 2-AB or procainamide.
Product documents:
Specsheet
Search for CofA
SDS:
CAB-STP-NEUAC-01
CPROC-STP-NEUAC-01
Part Numbers:
CAB-STP-NEUAC-01 Sialidase Testing Panel, 2-AB Labeled
CPROC-STP-NEUAC-01 Sialidase Testing Panel, Procainamide Labeled
recombinant from A. ureafaciens
α (2-3,6,8,9) Neuraminidase cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. The relative cleavage rates for different linkages are:
α(2-6) > α(2-3) > α(2-8), α(2-9).
View product documentation:
Specsheet
CofA
SDS: US EU
Part Number – Amount of Enzyme
E-S001 – 60 µLs¹
E-S001-20 – 20 µLs¹
E-S001-200 – 200 µls²
¹ includes buffer
² includes enzyme only
Neuraminidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.
recombinant from C. perfringens
View product documentation:
Specsheet
CofA
SDS: US EU
Part Number – Amount of Enzyme
E-S005 – 60 µLs¹
E-S005-20 – 20 µLs¹
E-S005-200 – 200 µls²
¹ includes buffer
² includes enzyme only
Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.
recombinant from Streptococcus pneumoniae in E. Coli
View product documentation:
Specsheet
CofA
SDS: US EU
Part Number – Amount of Enzyme
E-S007 – 60 µLs¹
E-S007-20 – 20 µLs¹
E-S007-200 – 200 µls²
¹ includes buffer
² includes enzyme only
The LudgerZyme Acetyl Esterase Kit removes 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins.
recombinant from Tannerella forsythia
View product documentation:
Specsheet/SDS
Search for CofA
Part Number
LZ-ACASE-KIT 50 µLs
The N-acetylneuraminic acid (NANA) standard is a quantitative standard of NIST-F and USP traceable Neu5Ac monosaccharide.
Product documents:
Certificate of Analysis Search
SDS
Part numbers:
CM-NEU-AC-01 1 nmols
CM-NEUAC-100 100 nmols
5-N-acetyl-9-O-acetyl neuraminic acid standard (Neu5,9Ac2)
Product documents:
Certificate of Analysis Search
SDS
~1 nmol (of which >750 pmol is Neu5,9Ac2)
Part Number: CM-NEU5,9AC2-01
The N-glycoylneuraminic acid (NGNA) standard is a quantitative standard of NIST-F and USP traceable Neu5Gc monosaccharide.
Product documents:
Certificate of Analysis Search
SDS
Part numbers:
CM-NEU-GC-01 1 nmols
CM-NEUGC-100 100 nmols
Certificate of Analysis Search
Certificate of Analysis Search
The Sialic Acid Reference panel is a mixture of different sialic acids forms (as detailed on chromatogram below) sourced from bovine submaxillary mucin.
It includes the following sialic acids: Neu5Ac; Neu5Gc; Neu5,7Ac2; Neu5,Gc9Ac; Neu5,9Ac2; Neu5,7,(8),9Ac3Gc.
Amount: approx. 1.25 nmols
Product documents:
Certificate of Analysis Search
SDS
Part Number: CM-SRP-01-C
The LudgerSep uR2 2.1x100mm column has been developed for UHPLC analysis of sialic acids labeled with DMB using the LudgerTag DMB Sialic Acid Labeling Kit.
Dimensions: 2.1mm x 100mm
View product documentation:
Product Specsheet/SDS
Certificate of Analysis Search
Part Number: LS-UR2-2.1×100