Sialic Acid Products

Sialic Acids (a family of derivatives of neuraminic acid) are usually found as terminal structures of both N-linked and O-linked glycans.

Being located at the terminal position of glycans, sialic acids are a likely point of contact for many glycoprotein interactions. They are also important for the stability and 3D conformation of glycoproteins and are involved in many biological interactions:

• Sialyation of IgG reduces ADCC and increases anti-inflammatory activity
• Sialic acids increase the serum half-life of glycoproteins by preventing uptake by the liver’s asialoglycoprotein receptor
• NGNA(Neu5Gc), a glycan not found in humans, can illicit an immune response and lead to increased neutralization of biopharmaceuticals.
• Cell line choice can greatly influence the type of neuraminic acids present on a biopharmaceutical, for example a large portion of the sialic acids on mouse IgG are often NGNA.

Sialidase Testing Panel

The Sialidase Testing Panel is a mixture of five different oligosaccharides containing NeuAc sialic acid linked in α2-3, α2-6, and α2-8 positions. The glycans are labeled with either 2-AB or procainamide.

Product documents:
Specsheet
Search for CofA
SDS:
CAB-STP-NEUAC-01
CPROC-STP-NEUAC-01

Part Numbers:
CAB-STP-NEUAC-01        Sialidase Testing Panel, 2-AB Labeled
CPROC-STP-NEUAC-01    Sialidase Testing Panel, Procainamide Labeled

recombinant from A. ureafaciens

α (2-3,6,8,9) Neuraminidase cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. The relative cleavage rates for different linkages are:
α(2-6) > α(2-3) > α(2-8), α(2-9).

View product documentation:
Specsheet
CofA

SDS: US   EU

Part Number – Amount of Enzyme
E-S001       – 60 µLs¹
E-S001-20   – 20 µLs¹
E-S001-200 – 200 µls²
¹ includes buffer
² includes enzyme only

Neuraminidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.

recombinant from C. perfringens

View product documentation:
Specsheet
CofA

SDS: US   EU

Part Number – Amount of Enzyme
E-S005       – 60 µLs¹
E-S005-20   – 20 µLs¹
E-S005-200 – 200 µls²
¹ includes buffer
² includes enzyme only

Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.

recombinant from Streptococcus pneumoniae in E. Coli

View product documentation:
Specsheet
CofA

SDS: US   EU

Part Number – Amount of Enzyme
E-S007       – 60 µLs¹
E-S007-20   – 20 µLs¹
E-S007-200 – 200 µls²
¹ includes buffer
² includes enzyme only

US$687

The LudgerZyme Acetyl Esterase Kit removes 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins.

recombinant from Tannerella forsythia

View product documentation:
Specsheet/SDS
Search for CofA

Part Number
LZ-ACASE-KIT       50 µLs

US$640

The LudgerTag DMB Sialic Acid Labeling Kit has been developed for the qualitative analysis of sialic acids.
The kit contains reagents for the release of sialic acids from glycoproteins. Released sialic acids are conjugated with DMB dye by an amination-cyclisation reaction.

The kit contains reagents and materials for the release and labeling of either 22 (LT-KDMB-A1) or 96 samples (LT-KDMB-96). The kit also includes the sialic acid reference panel, and the N-acetyl neuraminic acid and N-glycolyl neuraminic acid standards.

Product documents:
Product Guide  (includes SDS)
Certificate of Analysis Search

LT-KDMB-A1 – 22 samples
LT-KDMB-96 – 96 samples

US$46

The Sialic Acid Reference panel is a mixture of different sialic acids forms (as detailed on chromatogram below) sourced from bovine submaxillary mucin.

It includes the following sialic acids: Neu5Ac; Neu5Gc; Neu5,7Ac2; Neu5,Gc9Ac; Neu5,9Ac2; Neu5,7,(8),9Ac3Gc.

Amount: approx. 1.25 nmols

Product documents:
Certificate of Analysis Search
SDS

Part Number: CM-SRP-01

US$506

This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
The resulting molecule can then be covalently attached to an oligosaccharide by sialyltransferase.

Product documents:
Specsheet
SDS

Part Number: E-CMP01

US$506

Converts sialic acid to N-acetylmannosamine & pyruvate. Sialic acids can then be quantitated by a coupled reaction with NADH and lactic dehydrogenase

Product documents:
Specsheet
CoA
SDS: US  EU

Part Number: E-ALD01

US$575

The SialiQuant Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA).

Product documents:
Specsheet
SDS

Part Number: KE-SIALIQ