Sialic Acid Products

Sialic Acids (a family of derivatives of neuraminic acid) are usually found as terminal structures of both N-linked and O-linked glycans.

Being located at the terminal position of glycans, sialic acids are a likely point of contact for many glycoprotein interactions. They are also important for the stability and 3D conformation of glycoproteins and are involved in many biological interactions:

• Sialyation of IgG reduces ADCC and increases anti-inflammatory activity
• Sialic acids increase the serum half-life of glycoproteins by preventing uptake by the liver’s asialoglycoprotein receptor
• NGNA(Neu5Gc), a glycan not found in humans, can illicit an immune response and lead to increased neutralization of biopharmaceuticals.
• Cell line choice can greatly influence the type of neuraminic acids present on a biopharmaceutical, for example a large portion of the sialic acids on mouse IgG are often NGNA.

Sialidase Testing Panel

Sialidase Testing Panel

The Sialidase Testing Panel is a mixture of five different oligosaccharides containing NeuAc sialic acid linked in α2-3, α2-6, and α2-8 positions. The glycans are labeled with either 2-AB or procainamide.

View product overview as PDF
Download Non-hazardous SDS

Part Numbers:
CAB-STP-NEUAC-01        Sialidase Testing Panel, 2-AB Labeled
CPROC-STP-NEUAC-01    Sialidase Testing Panel, Procainamide Labeled

Neuraminidase Au image

Neuraminidase Au

recombinant from A. ureafaciens

α (2-3,6,8,9) Neuraminidase cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. The relative cleavage rates for different linkages are:
α(2-6) > α(2-3) > α(2-8), α(2-9).

View product documentation:
Specsheet    CoA    SDS

Part Number – Amount of Enzyme
E-S001       – 60 µLs¹
E-S001-20   – 20 µLs¹
E-S001-200 – 200 µls²
   ¹ includes buffer
   ² includes enzyme only

Neuraminidase Cp image

Neuraminidase Cp

Neuraminidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.

recombinant from C. perfringens

View product documentation:
Specsheet    CoA    SDS

Part Number – Amount of Enzyme
E-S005       – 60 µLs¹
E-S005-20   – 20 µLs¹
E-S005-200 – 200 µls²
   ¹ includes buffer
   ² includes enzyme only

Neuraminidase Sp image

Neuraminidase Sp

Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.

recombinant from Streptococcus pneumoniae in E. Coli

View product documentation:
Specsheet   CoA    SDS

Part Number – Amount of Enzyme
E-S007       – 60 µLs¹
E-S007-20   – 20 µLs¹
E-S007-200 – 200 µls²
   ¹ includes buffer
   ² includes enzyme only

Acetyl Esterase Kit

Sialate O-acetylesterase kit

$661

The LudgerZyme Acetyl Esterase Kit removes 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins.

recombinant from Tannerella forsythia

View product documentation:
Specsheet    CoA   SDS

Part Number
LZ-ACASE-KIT       50 µLs

Sialic acid labeling kit HPLC chromatogram and DMB dye diagram

DMB Sialic Acid Labeling Kit

$669

The LudgerTag DMB Sialic Acid Labeling Kit has been developed for the qualitative analysis of sialic acids.
The kit contains reagents for the release of sialic acids from glycoproteins. Released sialic acids are conjugated with DMB dye by an amination-cyclisation reaction.

The kit contains reagents and materials for up to 22 samples including the sialic acid reference panel, and the N-acetyl neuraminic acid and N-glycolyl neuraminic acid standards.

View product documentation as PDF
View Certificate of Analysis as PDF

Part Number: LT-KDMB-A1

Sialic Acid Aldolase

$506

Converts sialic acid to N-acetylmannosamine & pyruvate. Sialic acids can then be quantitated by a coupled reaction with NADH and lactic dehydrogenase

View product documentation:
Specsheet    CoA   SDS

Part Number: E-ALD01