Tools for Glycan Analysis
A mixtures of two glycopeptides with either F(6)A2 glycan or a F(3)A2 glycan attached to the asparagine amino acid of a peptide with the sequence Lysine-Valine-Alanine-Asparagine-Lysine-Threonine (KVANKT).
m/z: 2104.9411
Amount: ~6 micrograms
Part number: GPEP-FA2-01
The Ludger Exoglycosidase Clean-up Plates are a high-throughput option optimized for the removal of enzymes after exoglycosidase digestion or sequencing of glycans. Use of these 96-well plates prevents the contamination of HPLC columns during subsequent chromatographic analysis.
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Contains one 96-well plate
Part Number: LC-EXO-96
The Ludger Exoglycosidase Clean-up Cartridges are optimised for removal of enzymes post-exoglycosidase digestion of glycans. Use of these spin columns will prevent contamination of HPLC columns during subsequent chromatographic analysis.
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contains 6 cartridges
Part Number: LC-EXO-A6
The LudgerClean A Cartridges have been developed for glycopeptide enrichment from a peptide/glycopeptide mixture after protease digestion.
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Contains 24 cartridges
Part Number: LC-A-24
The Ludger EC50 plates have been developed as a high throughput option to purify glycans from complex mixtures including proteins, salts, and detergents.
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Contains one 96-well plate
Part Number: LC-EC50-96
The LudgerClean EC50 cartridges have been developed as a lower cost replacement for the EB10 cartridges while having equivalent efficiencies and reproducibility. Purify glycans from complex mixtures including proteins, salts, and detergents.
Applications include cleanup of glycans following hydrazinolysis, endoglycosidase digests (including PNGase F digests), enzyme treatment, and before and after fluorescent labeling.
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contains 24 cartridges
Part Number: LC-EC50-24
The Ludger Procainamide Cleanup Plate removes excess procainamide label after labeling of glycans. Excess procainamide interferes with subsequent sample analysis.
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Part Number: LC-PROC-96
This quantitative glycopeptide standard was developed for use during the sialic acid or monosaccharide release and labeling process. This will enable you to check the efficiency of glycan release, labeling, and recovery.
View the BioQuant Presentation
Purity: >90% as determined by UHPLC
Monoisotopic mass: 2865.1763 [M+H]+
Amount: 10 micrograms
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Download Non-hazardous SDS
Part number: BQ-GPEP-A2G2S2-10U
Procainamide labeling permits glycan identification by either mass spectrometry or UHPLC. Because of its improved ionisation efficiency compared to 2-AB labelling, it can permit identification of minor glycans (1% relative peak area) by ESI-MS.
View Presentation as PDF
View LT-KPROC-24 product documentation as PDF
View LT-KPROC-96 product documentation as PDF
View LT-KPROC-VP-24 product documentation as PDF
Search for Certificate of Analysis as PDF
Part Number Reductant
LT-KPROC-24 Sodium Cyanoborohydride
LT-KPROC-96 Sodium Cyanoborohydride
LT-KPROC-VP24 2-Picoline Borane
This kit is used for the permethylation of glycans. Permethylation stabilizes sialic acids for MALDI mass spectrometry analysis, and aids linkage analysis studies (MALDI-MS, ESI-MS).
– Improves and enhances ionization efficiency of glycans on mass spec
– Increased glycan hydrophobicity enables LC-MS analysis
– Relative and absolute quantitation can be performed
– Stabilizes the sialic acid moieties
– Easier determination of branching and glycosidic linkage positions
– Enables detection of both neutral and acidic glycans in positive ion mode
Photo Guide of the Procedure
View Product Documentation/SDS as PDF
View Certificate of Analysis as PDF
Part Number Reductant.
LT-PERMET-VP96 2-Picoline Borane – 96 samples
The BioQuant quantitative Man8 glycan is a purified and quantified glycan standard which can be used as an internal standard and positive control for quantitative analysis (e.g. monosaccharide analysis).
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Download Non-hazardous SDS
Glycan Purity determined as > 90% by UHPLC
Monoisotopic mass: 1721.6 [M+H]+
Part number: BQ-CN-MAN8-10U