QA-Bio supplies a full line of LudgerSep HPLC columns and buffers developed specifically for glycan analysis.
Normal phase – resolves glycans based on hydrophilicity, which is determined primarily by size, as well as arm specificity and linkage. Used for glycoprofiling of LudgerTag 2-AA and 2-AB labeled glycans showing the relative amount and size of all glycans released from the glycoprotein.
Anion exchange – LudgerSep columns and buffer for charge profile analysis of 2-AA and 2-AB labeled glycans. The number of sialic acids per glycan is the primary factor in the analysis.
Reverse phase – Ludger columns and buffers for the analysis of DMB labeled sialic acids and 2-AA labeled monosaccharides, with separation based on hydrophobicity.