LudgerTag procainamide labeled glycans can be analyzed by UHPLC, HPLC, ESI-MS, and LC-ESI-MS methods. Because of its improved ionization efficiency compared to 2-AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.
Fluorescence response: Procainamide glycans show a much higher sensitivity as compared to 2-AB labeled glycans, providing peak heights three times higher than traditional 2-AB labeled glycans. The procainamide labeled sugars show comparable separation to HILIC-UHPLC assays with 2-AB.
Mass spectrometry response: Due to its high ionization efficiency, procainamide shows much higher sensitivities the seen with 2-AB. When analyzed using ESI-MS and ESI-MS/MS, procainamide produces twenty-two times higher peaks than 2-AB. This provides a great platform for the detection and identification on minor peaks by MS/MS analysis.
Mass free dye = 235.33.
Fluorescence, λex = 310 nm, λem = 370 nm.
The labeled glycans are supplied as 20 pmols dried form from an aqueous solution. They are stable at least 5 years as supplied and after reconstitution.
HPLC Calibration Standard
Glucose homopolymer ladder derived from digested wheat starch used as a standard for sizing of glycans.
Part Number Amount Label
CAB-GHP-30 100 picomoles 2-AB
CAA-GHP-30 100 picomoles 2-AA
CPROC-GHP-30 100 picomoles Procainamide