LudgerTag kit reagents are purified to analytical grade and are dispensed and sealed under clean, inert atmospheres. The ampoules are pre-cleaned by pyrolysis at 500°C then opened just before dispensing and sealing under oxygen-free dry nitrogen. These controls ensure that your analyses work properly each and every time.
Acetic Acid – Promotes acid catalyzed ring opening of the glycan reducing terminus prior to the formation of an imine intermediate between the sugars and the unprotonated form of the dye
Fluorescent Dye – The comprehensive range of LudgerTag dyes include 2-AB, 2-AA, Procainamide, DMB, and APTS to cover different types of glycoanalyses by HPLC, CE, and MS.
DMSO – An anhdrous solvent used to dissolve all the components of the reductive amination labeling reaction.
Sodium Cyanoborohydride or Non-Toxic 2PB – This reductant stabilizes the imine produced by the reaction of the dye and glycans to produce stable fluorescently labeled glycan
2-AA (2-aminobenzoic acid) is considered by many to be a superior replacement for 2-AB (2-aminobenzamide) in most types of complex glycan analysis. 2-AA is reported to have a higher fluorescence and gives higher labelling efficiencies than 2-AB.
Part Number: LT-KAA-A2
The LudgerTag DMB Labeling Kit has been developed for the qualitative analysis of sialic acids
The kit contains two sets of the labeling reagents and a sialic acid reference panel (pictured):
- Sialic Acid Reference Panel
- DMB Dye (1,2-diamino-4,5-methylenedioxybenzene.2HCl)
- Acetic Acid
- Mercaptoethanol in Acetic acid
- Sodium Dithionite (Reductant)
Typically, up to 15 separate analytical samples can be labeled per set.
Part Number: LT-KDMB-A1
Procainamide labelling permits glycan identification by either mass spectrometry or UHPLC. Because of its improved ionisation efficiency compared to 2AB labelling, it can permit identification of minor glycans (1% relative peak area) by ESI-MS. This LudgerTag kit, along with a post-labelling clean up plate for the samples (catalog# LC-PROC-96), has been validated in house at Ludger. Typical CVs for triplicate analyses were 5%
Part Number: LT-KPROC-VP24
The LudgerTag V-tag labeling kit is designed for the fluorophore labeling of enzymatically digested peptides and glycopeptides followed by enrichment of the labeled glycopeptides.
Part Number: LT-VTAG-24
This kit is used for the permethylation of glycans. Permethylation stabilizes sialic acids for MALDI mass spectrometry analysis, and aids linkage analysis studies (MALDI-MS, ESI-MS).
- Improves and enhances ionization efficiency of glycans on mass spec
- Increased glycan hydrophobicity enables LC-MS analysis
- Relative and absolute quantitation can be performed
- Stabilizes the sialic acid moieties
- Easier determination of branching and glycosidic linkage positions
- Enables detection of both neutral and acidic glycans in positive ion mode
Part Number: LT-PERMET-VP96