Glycan Labeling

LudgerTag Glycan Labeling Kit reagents are purified to analytical grade and are dispensed and sealed under clean, inert atmospheres. The ampoules are pre-cleaned by pyrolysis at 500°C then opened just before dispensing and sealing under oxygen-free dry nitrogen. These controls ensure that your analyses work properly each and every time.

Other “instant” labeling systems label the amino group formed during PNGase f release before hydrolysis of the reducing terminal of the sugar. This means these systems cannot be used to label any pre-released glycan standards or other sugars with free reducing ends such as O-glycans, GSL-glycans, or GAGS.

2-AB and 2-AA are used in HILIC-(U)HPLC, WAX-HPLC, and MALDI-MS assays. They are the most referenced glycan label for biopharmaceutical analysis. 2-AA is also used for monosaccharide analysis.

DMB labeled sialic acids are used in the qualitative analysis of sialic acids

Procainamide labeled glycans are commonly used in HILIC-(U)HPLC and ESI-MS assays. Procainamide gives >3 times more response in HPLC assays compared to 2-AB labelled glycans and >30 times more response for ESI-MS.

Permethylation stabilizes sialic acids for MALDI-MS techniques and aids linkage analysis studies (MALDI-MS, ESI-MS).

Procainamide Labeling Kit

Procainamide Labeling Kit

Procainamide labeling permits glycan identification by either mass spectrometry or UHPLC. Because of its improved ionisation efficiency compared to 2-AB labelling, it can permit identification of minor glycans (1% relative peak area) by ESI-MS.

View procainamide analysis presentation as PDF

View Product documentation- includes SDS:
LT-KPROC-24
LT-KPROC-96
LT-KPROC-VP-24
Certificate of Analysis Search

Part Number           Reductant
LT-KPROC-24        Sodium Cyanoborohydride
LT-KPROC-96        Sodium Cyanoborohydride
LT-KPROC-VP24   2-Picoline Borane

permethylation kit image

Permethylation Kit

US$854

This kit is used for the permethylation of glycans. Permethylation stabilizes sialic acids for MALDI mass spectrometry analysis, and aids linkage analysis studies (MALDI-MS, ESI-MS).

– Improves and enhances ionization efficiency of glycans on mass spec
– Increased glycan hydrophobicity enables LC-MS analysis
– Relative and absolute quantitation can be performed
– Stabilizes the sialic acid moieties
– Easier determination of branching and glycosidic linkage positions
– Enables detection of both neutral and acidic glycans in positive ion mode

Photo Guide of the Procedure
Product Guide  (includes SDS)
Certificate of Analysis Search

Part Number        Reductant.
LT-PERMET-VP96   2-Picoline Borane – 96 samples

2-AB Glycan labeling kit image

2-AB Labeling Kits

2-AB (2-aminobenzamide) is one of the most widely used fluorescent labels for glycosylation analysis. The LudgerTag 2-AB Labeling Kit includes all the reagents required to label up to 30 glycans samples.

Two kits are available which differ in the type of reductant supplied: sodium cyanoborohydride or non-toxic 2-picoline borane.

Part Number    Reductant
LT-KAB-A2       Sodium Cyanoborohydride – 24 samples
LT-KAB-VP24   2-Picoline Borane – 24 samples

LT-KAB-VP96   2-Picoline Borane – 96 samples

View product documents:
Photo Guide of the Procedure
LT-KAB-A2 (includes SDS)
LT-KAB-VP26 (includes SDS)
Certificate of Analysis Search

2-AA labeling kit image

2-AA Labeling Kit

2-AA (2-aminobenzoic acid) is considered by many to be a superior replacement for 2-AB in most types of complex glycan analysis. 2-AA labeling is reported to have a higher fluorescence and gives higher labelling efficiencies than 2-AB.

Two kits are available which differ in the type of reductant supplied: sodium cyanoborohydride or non-toxic 2-picoline borane.

Part Number    Reductant
LT-KAA-A2       Sodium Cyanoborohydride
LT-KAA-VP24   2-Picoline Borane

View product documents:
Protocol picture guide
LT-KAA-A2 (includes SDS)
LT-KAA-VP24 (includes SDS)
Certificate of Analysis Search

Sialic acid labeling kit HPLC chromatogram and DMB dye diagram

DMB Sialic Acid Labeling Kit

The LudgerTag DMB Sialic Acid Labeling Kit has been developed for the analysis of sialic acids.  We have both qualitative and quantitative kits.

LT-KDMB-12 – Qualitative kit for up to 12 samples. Includes a sialic acid reference panel to identify sialic acid profiles
Product Guide    (includes SDS)

Certificate of Analysis Search

LT-KDMB-A1 and LT-KDMB-96 – Quantitative sialic acid analysis for 24 and 96 samples. Includes the sialic acid reference panel and two quantitative standards, N-acetyl neuraminic acid and N-glycolyl neuraminic acid.
Product Guide (includes SDS)

Certificate of Analysis Search

 

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