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Glycan Labeling

LudgerTag Glycan Labeling Kit reagents are purified to analytical grade and are dispensed and sealed under clean, inert atmospheres. The ampoules are pre-cleaned by pyrolysis at 500°C then opened just before dispensing and sealing under oxygen-free dry nitrogen. These controls ensure that your analyses work properly each and every time.

Other “instant” labeling systems label the amino group formed during PNGase f release before hydrolysis of the reducing terminal of the sugar. This means these systems cannot be used to label any pre-released glycan standards or other sugars with free reducing ends such as O-glycans, GSL-glycans, or GAGS.

2-AB and 2-AA are used in HILIC-(U)HPLC, WAX-HPLC, and MALDI-MS assays. They are the most referenced glycan label for biopharmaceutical analysis. 2-AA is also used for monosaccharide analysis.

The DMB Sialic Acid Labeling Kit has been developed for the qualitative analysis of sialic acids

Procainamide labeled glycans are commonly used in HILIC-(U)HPLC and ESI-MS assays. Procainamide gives >3 times more response in HPLC assays compared to 2-AB labelled glycans and >30 times more response for ESI-MS.

Permethylation stabilizes sialic acids for MALDI-MS techniques and aids linkage analysis studies (MALDI-MS, ESI-MS).

Glycan Labeling Kit Components

Acetic Acid – Promotes acid catalyzed ring opening of the glycan reducing terminus prior to the formation of an imine intermediate between the sugars and the unprotonated form of the dye

Fluorescent Dye – The comprehensive range of LudgerTag dyes include 2-AB, 2-AA, Procainamide, DMB, and APTS to cover different types of glycoanalyses by HPLC, CE, and MS.

DMSO – An anhdrous solvent used to dissolve all the components of the reductive amination labeling reaction.

Sodium Cyanoborohydride or Non-Toxic 2PB – This reductant stabilizes the imine produced by the reaction of the dye and glycans to produce stable fluorescently labeled glycan

Procainamide Image

Procainamide Labeling Kit

Procainamide labeling permits glycan identification by either mass spectrometry or UHPLC. Because of its improved ionisation efficiency compared to 2-AB labelling, it can permit identification of minor glycans (1% relative peak area) by ESI-MS.

Photo Guide of the Procedure

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number        Reductant
LT-KPROC-24       Sodium Cyanoborohydride
LT-KPROC-VP24   2-Picoline Borane

permethylation kit image

Permethylation Kit

This kit is used for the permethylation of glycans. Permethylation stabilizes sialic acids for MALDI mass spectrometry analysis, and aids linkage analysis studies (MALDI-MS, ESI-MS).

- Improves and enhances ionization efficiency of glycans on mass spec
- Increased glycan hydrophobicity enables LC-MS analysis
- Relative and absolute quantitation can be performed
- Stabilizes the sialic acid moieties
- Easier determination of branching and glycosidic linkage positions
- Enables detection of both neutral and acidic glycans in positive ion mode

Photo Guide of the Procedure
View product documentation as PDF
View Certificate of Analysis as PDF

Part Number        Reductant
LT-PERMET-VP24   2-Picoline Borane

2-AB labeling kit image

2-AB Labeling Kits

2-AB (2-aminobenzamide) is one of the most widely used fluorescent labels for glycosylation analysis. The LudgerTag 2-AB Labeling Kit includes all the reagents required to label up to 30 glycans samples.Photo Guide of the Procedure View product documentation as PDF View Certificate of Analysis as PDFTwo kits are available which differ in the type of reductant supplied: sodium cyanoborohydride or non-toxic 2-picoline borane.Part Number    Reductant LT-KAB-A2       Sodium Cyanoborohydride LT-KAB-VP24   2-Picoline Borane
2-AA labeling kit image

2-AA Labeling Kit

2-AA (2-aminobenzoic acid) is considered by many to be a superior replacement for 2-AB in most types of complex glycan analysis. 2-AA labeling is reported to have a higher fluorescence and gives higher labelling efficiencies than 2-AB.View protocol picture guide as a PDF View product documentation as PDF View Certificate of Analysis as PDFTwo kits are available which differ in the type of reductant supplied: sodium cyanoborohydride or non-toxic 2-picoline borane.Part Number    Reductant LT-KAA-A2       Sodium Cyanoborohydride LT-KAA-VP24   2-Picoline Borane
Monosaccharide Analysis Kit Image

Monosaccharide Analysis Kit

The LudgerTag Monosaccharide Analysis Kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards.

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-MONO-96

$1,313.00
Sialic acid labeling kit HPLC chromatogram and DMB dye diagram

DMB Sialic Acid Labeling Kit

The LudgerTag DMB Sialic Acid Labeling Kit has been developed for the qualitative analysis of sialic acids

The kit contains two sets of the labeling reagents and a sialic acid reference panel (pictured).

Typically, up to 15 separate analytical samples can be labeled per set.

View product documentation as PDF
View Certificate of Analysis as PDF

Part Number: LT-KDMB-A1

V-Tag glycopeptide labeling kit image

V-Tag Glycopeptide Labeling Kit

The LudgerTag V-tag labeling kit is designed for the fluorophore labeling of enzymatically digested peptides and glycopeptides followed by enrichment of the labeled glycopeptides. The V-tag labeled glycopeptides are analyzed using MALDI-MS and HPLC/UHPLC

Photo Guide of the Procedure
View product documentation as PDF
View Certificate of Analysis as PDF

Part Number: LT-VTAG-24

$1,184.00
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