Glycan Purification
Glycan purification is an essential technology for glycoprofiling. Salts, detergents, proteins, and other contaminants can interfere with fluorescent labeling of released glycans. Exo- and endoglycosidases can contaminate HPLC columns and interfere with mass spectrometry methods for the analysis of glycans.
A number of technologies have been developed for the purification of glycans from proteins, salts, and detergents by our partners at Ludger Ltd:
LudgerClean EB10 cartridges have been designed for purification of glycans from proteins, salts, and detergents. The cartridges contain a unique graphitised carbon material which acts like a super-hydrophobic resin that binds even very hydrophilic glycans.
The LudgerClean PBM Post-Glycosidase Clean-Up plate has been developed for removal of glycosidases prior to MS analysis in a 96-well format.
LudgerClean CEX cartridges purify O-glycans from non-carbohydrate material including salts, proteins, and detergents. The cartridge contains a cation exchange resin.
LudgerClean Pre-permethylation Plates are used for the removal of excess salts, detergent, proteins and other impurities after the release of N-glycans from glycoproteins. This clean-up plate is used to enrich N-glycans prior to performing permethylation of released glycans.
EB10 Cartridges
Ludger EB10 cartidges have been designed for purification of glycans from proteins, salts, and detergents.
Applications include cleanup of glycans following hydrazinolysis, endoglycosidase digests (including PNGase F digests), enzyme treatment, and before and after fluorescent labeling.
View product documentation as PDF
View Certificate of Analysis as PDF
contains 6 cartridges
Part Number: LC-EB10-A6
PBM Glycosidase Removal
The LudgerClean PBM Glycosidase removal plate was developed for the capture of endo- and exoglycosidases after deglycosylation to prevent contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove glycosidases or other proteins before mass spectrometry analysis of glycans.
View product documentation as PDF
View Certificate of Analysis as PDF
Part Number: LC-PBM-96
CEX Cartridges for O-glycans
For the purification of O-glycans from non-carbohydrate material including salts, proteins, and detergents. Applications include cleanup of glycans following hydrazinolysis or any other non-reductive O-glycan release method
View product documentation as PDF
View Certificate of Analysis as PDF
contains 6 cartridges
Part Number: LC-CEX-A6
Pre-Permethylation Cleanup Plate
The LudgerClean Pre-Permethylation Cleanup Plate is used to enrich N-glycans prior to performing permethylation of released glycans. The 96 well plate is used for removal of excess salts, detergent, proteins and other impurities after the release of N-glycans from glycoproteins.
View product documentation as PDF
View Certificate of Analysis as PDF
Part Number: LC-PERMET-96
EC50 Cartridges
The LudgerClean EC50 cartridges have been developed as a lower cost replacement for the EB10 cartridges while having equivalent efficiencies and reproducibility.
Applications include cleanup of glycans following hydrazinolysis, endoglycosidase digests (including PNGase F digests), enzyme treatment, and before and after fluorescent labeling.
View product documentation as PDF View Certificate of Analysis as PDF
contains 24 cartridges
Part Number: LC-EC50-24