Glycan purification is an essential technology for glycoprofiling. Salts, detergents, proteins, and other contaminants can interfere with fluorescent labeling of released glycans. Exo- and endoglycosidases can contaminate HPLC columns and interfere with mass spectrometry methods for the analysis of glycans.
A number of technologies have been developed for the purification of glycans from proteins, salts, and detergents by our partners at Ludger Ltd:
LudgerClean EC50 cartridges have been developed as a lower cost replacement for the EB10 cartridges while having equivalent efficiencies and reproducibility. Removes proteins, salts, and detergents.
LudgerClean EB10 cartridges have been designed for purification of glycans from proteins, salts, and detergents. The cartridges contain a unique graphitised carbon material which acts like a super-hydrophobic resin that binds even very hydrophilic glycans.
LudgerClean CEX cartridges purify O-glycans from non-carbohydrate material including salts, proteins, and detergents. The cartridge contains a cation exchange resin.