Glycan purification is an essential technology for glycoprofiling. Salts, detergents, proteins, and other contaminants can interfere with fluorescent labeling of released glycans. Exo- and endoglycosidases can contaminate HPLC columns and interfere with mass spectrometry methods for the analysis of glycans.
A number of technologies have been developed for the purification of glycans from proteins, salts, and detergents by our partners at Ludger Ltd:
LudgerClean EB10 cartridges have been designed for purification of glycans from proteins, salts, and detergents. The cartridges contain a unique graphitised carbon material which acts like a super-hydrophobic resin that binds even very hydrophilic glycans.
The LudgerClean PBM Post-Glycosidase Clean-Up plate has been developed for removal of glycosidases prior to MS analysis in a 96-well format.
LudgerClean CEX cartridges purify O-glycans from non-carbohydrate material including salts, proteins, and detergents. The cartridge contains a cation exchange resin.
LudgerClean Pre-permethylation Plates are used for the removal of excess salts, detergent, proteins and other impurities after the release of N-glycans from glycoproteins. This clean-up plate is used to enrich N-glycans prior to performing permethylation of released glycans.