Glycan Purification

Glycan purification is an essential technology for glycoprofiling. Salts, detergents, proteins, and other contaminants can interfere with fluorescent labeling of released glycans. Exo- and endoglycosidases can contaminate HPLC columns and interfere with mass spectrometry methods for the analysis of glycans.

A number of technologies have been developed for the purification of glycans from proteins, salts, and detergents by our partners at Ludger Ltd:

Exoglycosidase Clean-up Plate are optimised for removal of enzymes post-exoglycosidase digestion of glycans. Use of these spin columns will prevent contamination of HPLC columns during subsequent chromatographic analysis.

Exoglycosidase Clean-up Cartridges are a high-throughput option for the removal of enzymes post-exoglycosidase digestion of glycans, preventing contamination of HPLC columns during subsequent chromatographic analysis.

A Cartridges have been developed for glycopeptide enrichment from a peptide/glycopeptide mixture after protease digestion.

EC50 96-Well Plate The Ludger EC50 plates are a high throughput option for the purification of glycans from complex mixtures including proteins, salts, and detergents.

EC50 cartridges have been developed as a lower cost replacement for the EB10 cartridges while having equivalent efficiencies and reproducibility. Removes proteins, salts, and detergents.

EB10 cartridges purify glycans from complex mixtures containing proteins, salts, and detergents. The cartridges contain a unique graphitised carbon material which acts like a super-hydrophobic resin that binds even very hydrophilic glycans.

CEX cartridges purify O-glycans from non-carbohydrate material including salts, proteins, and detergents. The cartridge contains a cation exchange resin.

LC-EXO-96 Exoglycosidase Clean-up Plate
US$265

The Ludger Exoglycosidase Clean-up Plates are a high-throughput option optimized for the removal of enzymes after exoglycosidase digestion or sequencing of glycans. Use of these 96-well plates prevents the contamination of HPLC columns during subsequent chromatographic analysis.

View product documentation:
Product Specsheet/SDS
Certificate of Analysis Search

Contains one 96-well plate

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Part Number: LC-EXO-96

US$940

The LudgerClean EC50 cartridges have been developed as a lower cost replacement for the EB10 cartridges while having equivalent efficiencies and reproducibility. Purify glycans from complex mixtures including proteins, salts, and detergents.

Applications include cleanup of glycans following hydrazinolysis, endoglycosidase digests (including PNGase F digests), enzyme treatment, and before and after fluorescent labeling.

View EC50 online presentation

View product documentation:
Product Specsheet/SDS
Certificate of Analysis Search

contains 24 cartridges

Part Number: LC-EC50-24

US$289

Ludger EB10 cartidges have been designed for purification of glycans from proteins, salts, and detergents.

Applications include cleanup of glycans following hydrazinolysis, endoglycosidase digests (including PNGase F digests), enzyme treatment, and before and after fluorescent labeling.

View product documentation:
Product Specsheet/SDS
Certificate of Analysis Search

contains 6 cartridges

Part Number: LC-EB10-A6