Exoglycosidases

recombinant from A. ureafaciens

α (2-3,6,8,9) Neuraminidase cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. The relative cleavage rates for different linkages are: α(2-6) > α(2-3) > α(2-8), α(2-9).

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Specsheet    CoA    MSDS

Part Number - Amount of Enzyme
E-S001       - 60 µLs¹
E-S001-20   - 20 µLs¹
E-S001-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

Neuraminidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.

recombinant from C. perfringens

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Specsheet    CoA    MSDS

Part Number - Amount of Enzyme
E-S005       - 60 µLs¹
E-S005-20   - 20 µLs¹
E-S005-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.

recombinant from Streptococcus pneumoniae in E. Coli

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Specsheet   CoA    MSDS

Part Number - Amount of Enzyme
E-S007       - 60 µLs¹
E-S007-20   - 20 µLs¹
E-S007-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

Beta Galactosidase from Streptococcus pneumoniae releases only β(1-4)- linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

recombinant from Streptococcus pneumoniae

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Specsheet    CoA   MSDS

Part Number - Amount of Enzyme
E-BG07       - 60 µLs¹
E-BG07-20   - 20 µLs¹
E-BG07-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

Beta-(1-3,4,6) Galactosidase cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose.

Purified from bovine testes

product documentation:
Specsheet    CoA   MSDS

Part Number: E-BG02

Alpha Galactosidase from E. coli cleaves α(1-3)- and α(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

recombinant from E. coli in E. coli

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Specsheet    CoA   MSDS

Part Number - Amount of Enzyme
E-AG02       - 60 µLs¹
E-AG02-20   - 20 µLs¹
E-AG02-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and glycoproteins.

recombinant from Streptococcus pneumoniae in E. Coli

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Specsheet    CoA   MSDS

Part Number - Amount of Enzyme
E-GL01       - 60 µLs¹
E-GL01-20   - 20 µLs¹
E-GL01-200 - 200 µls²
¹ includes buffer
² includes enzyme only

Alpha Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.

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Specsheet    CoA   MSDS

Part Number - Amount of Enzyme
E-AM01       - 60 µLs¹
E-AM01-20   - 20 µLs¹
E-AM01-200 - 200 µls²
   ¹ includes buffer
   ² includes enzyme only

Alpha-(1-6) Core Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides.

recombinant from Xanthamonas manihotis E. Coli

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Specsheet    CoA   MSDS

Part Number - Amount of Enzyme
E-AM02       - 60 µLs¹
E-AM02-20   - 20 µLs¹
E-AM02-200 - 200 µls²
¹ includes buffer
² includes enzyme only

Removes non-reducing, terminal branched fucose when linked alpha-(1-6) to the core GlcNAc of a GlcNAc-GlcNAc disaccharide structure that has been labeled with a fluorescent molecule. To date, the fluorescent labels ANTS, ANDSA and 2-AA (LT-KAA-A2) have been demonstrated to function with the fucosidase. recombinant Elizabethkingia meningosepticum View product documentation:
Specsheet    CoA   MSDS Part Number - Amount of Enzyme
E-F006       - 60 µLs¹
E-F006-20   - 20 µLs¹
E-F006-200 - 200 µls²
¹ includes buffer
² includes enzyme only

Alpha (1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures.

Xanthamonas manihotis

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Specsheet    CoA   MSDS

Part Number - Amount of Enzyme
E-F134       - 60 µLs¹
E-F134-20   - 20 µLs¹
E-F134-200 - 200 µls²
¹ includes buffer
² includes enzyme only

Our Glycan Sequencing Kit includes the enzymes, and buffers required to sequence ten N-linked oligosaccharides

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Specsheet    CoA   MSDS

Part Number: KE-SQ01