Endoglycosidases

-Endoglycosidases cleave intact glycans from glycoprotein
-Highly stable enzymes in clean preparations- no glycerol, NaCl, or other additives such as EDTA.
-All enzymes are tested for the absence of proteolytic or unexpected glycosidic activity

Endoglycosidases are some of the most widely used enzymes for the analysis of glycoproteins. These enzymes release intact glycans from glycoproteins, while exoglycosidases release individual monosaccharides (i.e. sialic acid, galactose, ß-N-acetylgucosamine, mannose, etc.).

PNGase F is commonly included in the group of enzymes due to its similar activity of cleaving intact N-linked glycans, though it is actually an amidase. It cleaves between the asparagine amino acid and the first ß-N-acetylglucosamine sugar (GlcNAc) of the chitobiose core of N-linked glycans. The Endo F enzymes and Endo H cleave N-linked glycans between the first and second GlcNAc, leaving a hydrophilic residue which aids in increasing solubility of the deglycosylated protein, reducing the potential for precipitation of the protein aggregates. PNGase F cleaves all N-linked glycans, while Endo H and the Endo F enzymes remove specific types of N-linked glycans. PNGase F will not remove oligosaccharides containing alpha-(1,3)-linked core fucose commonly found on plant glycoproteins.

Also included in this classification of enzymes is O-Glycosidase, which removes the Core 1 O-Linked glycan (Gal-β1,3-GalNAc) from serine or threonine amino acids. Often times O-linked glycans contain additional monosaccharides linked to the galactose in both branched and linear linkages, requiring the use of exoglycosidases such as sialidase and galactosidase to remove the additional sugars.

PNGase F image

PNGase F

PNGase F cleaves N-linked (asparagine-linked) oligosaccharides from glycoproteins.

Enzyme remains fully active for at least 96 hours at 37˚C.

View product documentation:
Specsheet   CofA  SDS

Part Number – Amount of Enzyme
E-PNG01         – 60 µLs¹
E-PNG01-20   –  20 µLs¹
E-PNG01-200 – 200 µls² (previously E-PNG05)
¹ includes buffer, denaturant, and Triton-X
² includes enzyme only

PNGase F image

Recombinant PNGase F

recombinant PNGase F from Elizabethkingia miricola

View product documentation:
Specsheet   CofA   SDS

Part Number – Amount of Enzyme
E-RPNG01     – 60 µLs¹
E-RPNG01-20   – 20 µls¹
E-RPNG01-200 – 200 µls²
¹ includes buffer, denaturant, and Triton-X
² includes enzyme only

Endo F1 image

Endo F1

Endo F1 cleaves high mannose and some hybrid type N-glycans from peptides and proteins

View product documentation:
Specsheet   CofA   SDS

Part Number – Amount of Enzyme
E-EF01     – 60 µLs¹
E-EF01-20   – 20 µls¹
E-EF01-200 – 200 µls²
  ¹ includes buffer
  ² includes enzyme only

Endo F2 image

Endo F2

Selective release of biantennary glycans from peptides and proteins

View product documentation:
Specsheet   CoA   SDS

Part Number – Amount of Enzyme
E-EF02     – 60 µLs¹
E-EF02-20   – 20 µls¹
E-EF02-200 – 200 µls²
¹ includes buffer
² includes enzyme only

Endo F3 image

Endo F3

Selective release of triantennarry and alpha-(1-6)-fucosylated biantennary N-glycans from peptides and protein

View product documentation:
Specsheet   CoA   SDS

Part Number – Amount of Enzyme
E-EF03     – 60 µLs¹
E-EF03-20   – 20 µls¹
E-EF03-200 – 200 µls²
  ¹ includes buffer
  ² includes enzyme only

Endo F Multi Kit image

Endo F Multi-Kit

$506

The Endo F Multi-Kit will deglycosylate N-linked glycans in both native and denatured conditions. Each enzyme has a distinct specificity for N-linked glycan release. One can choose to use the three enzymes in combination to completely remove all N-linked glycans present on a glycoprotein or peptide, or to use each enzyme independently and thereby determine the type of N-glycans present.

View product documentation:
Specsheet   CofA  SDS

Part Number: KE-EFX3

Endo H image

Endo-H

Endo H cleaves asparagine-linked hybrid or high mannose oligosaccharides but not complex oligosaccharides

View product documentation:
Specsheet   CoA   SDS

Part Number – Amount of Enzyme
E-EH02     – 60 µLs¹
E-EH02-20   – 20 µls¹
E-EH02-200 – 200 µls²
¹ includes buffer and denaturant
² includes enzyme only

O-Glycosidase image

O-Glycosidase

recombinant Streptococcus pneumoniae

View product documentation:
Specsheet   CoA   SDS

Part Number – Amount of Enzyme
E-G001     – 60 µLs¹
E-G001-20   – 20 µls¹
E-G001-200 – 200 µls²
¹ includes buffer
² includes enzyme only

Endo Beta Galactosidase image

Endo-Beta-Galactosidase

Endo-beta-galactosidase cleaves internal ß(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures.

Recombinant from Bacteroides fragili.

View product documentation:
Specsheet   CoA   SDS

Part Number – Amount of Enzyme
E-XBG01     – 60 µLs¹
E-XBG01-20   – 20 µls¹
E-XBG01-200 – 200 µls²
  ¹ includes buffer
  ² includes enzyme only

Ceramide Glycanase

$387

LudgerZyme Ceramide Glycanase

Ceramide glycanase can be used to deglycosylate a variety of glycosphingolipids by cleaving the β-glycosyl linkage.

View product documentation:
Specsheet   CofA  SDS

Part number: LZ-CER-HM-KIT