QA-Bio provides easy to use deglycosylation kits for removing N-linked and O-linked glycans from glycoproteins in both native & denatured conditions.
PNGase F is commonly used to deglycosylate glycoproteins containing only N-linked glycans, working in both denatured and native conditions. For native samples that are slowly cleaved by PNGase F or those that precipitate after PNGase F digestion, our Endo F Multi-Kit is recommended. Both methods leave the glycans intact and available for further analysis.
If the glycan type is unknown, or if it consists of both N-linked & O-linked glycans, the use of our Enzymatic CarboRelease Kit or our Enzymatic DeGlycoMx Kit is recommended. These deglycosylation kits include a number of enzymes which will remove and digest the glycans into monosaccharides. In the DeglycoMx Kit, the enzymes are packaged as a premixed cocktail of enzymes in one 20 microlitre vial. For the CarboRelease Kit, the enzymes are supplied separately in individual vials of 20 microlitres, allowing greater flexibility in assay design.
The LudgerLiberate Hydrazinolysis Kit contains reagents for the release of N and O-linked glycans from glycoprotein biopharmaceuticals. Released glycans have free reducing terminii to allow fluorescent tagging by reductive amination. The release conditions can be optimized for release of N-glycans, O-glycans or both N- and O-glycans.
For release and recovery of O-linked Glycans, we recommend our Ludger Orela kit.
The Ludger Liberate Orela Kit has been developed for O-linked deglycosylation of glycoproteins. Released glycans have free reducing terminii to allow fluorescent tagging by reductive amination.
Part Number: LL-ORELA-A2
Ludger Liberate Membrane Glycan Release kit is used for immobilizing, clean-up and PNGaseF mediated release of glycans from glycoproteins.
The ability to immobilize glycoproteins allows the removal of low molecular weight solvent/buffer components, such as trehalose, which may interfere with glycan analysis.
Part Number: LL-MR-A2