Tools for Glycan Analysis
PNGase F is commonly used to deglycosylate glycoproteins containing only N-linked glycans, working in both denatured and native conditions. For native samples that are slowly cleaved by PNGase F or those that precipitate after PNGase F digestion, our Endo F Multi-Kit is recommended. Both methods leave the glycans intact and available for further analysis.
If the glycan type is unknown, or if it consists of both N-linked & O-linked glycans, the use of our Enzymatic CarboRelease Kit or our Enzymatic DeGlycoMx Kit is recommended. These deglycosylation kits include a number of enzymes which will remove and digest the glycans into monosaccharides. In the DeglycoMx Kit, the enzymes are packaged as a premixed cocktail of enzymes in one 20 microlitre vial. For the CarboRelease Kit, the enzymes are supplied separately in individual vials of 20 microlitres, allowing greater flexibility in assay design.
The LudgerLiberate Hydrazinolysis Kit contains reagents for the release of N and O-linked glycans from glycoprotein biopharmaceuticals. Released glycans have free reducing terminii to allow fluorescent tagging by reductive amination. The release conditions can be optimized for release of N-glycans, O-glycans or both N- and O-glycans.
For release and recovery of O-linked Glycans, we recommend our Ludger Orela kit.
The Endo F Multi-Kit will deglycosylate N-linked glycans in both native and denatured conditions. Each enzyme has a distinct specificity for N-linked glycan release. One can choose to use the three enzymes in combination to completely remove all N-linked glycans present on a glycoprotein or peptide, or to use each enzyme independently and thereby determine the type of N-glycans present.
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Specsheet
CofA
SDS: US EU
Part Number: KE-EFX3
This kit includes the enzymes and buffers required to deglycosylate glycoproteins, removing all N-linked oligosaccharides and many O-linked sugars.
The enzymes are packaged separately in individual 20 microlitre vials. This gives researchers the flexibility to characterize the glycans attached to their glycoprotein more fully than with our mixture of enzymes, KE-DGMX.
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Specsheet
CofA
SDS: US EU
Part Number: KE-DG01
This kit developed for protein deglycosylation includes our DeGlycoMx, a premixed cocktail of the enzymes required to remove all N-linked oligosaccharides and most O-linked sugars from 0.5 mg of glycoprotein, via 10 reactions of up to 50 micrograms of protein per reaction.
The kit is easy-to-use and effective: add 2 µl of the DeGlycoMx enzyme to your denatured sample and the included buffer and incubate for 3 hours at 37˚C.
View product documentation:
Specsheet
CofA
SDS: US EU
Part Number:
KE-DGMX – 20 microliters
KE-DGMX-100 – 100 microliters
The Ludger Liberate Orela Kit has been developed for O-linked deglycosylation of glycoproteins. Released glycans have free reducing terminii to allow fluorescent tagging by reductive amination.
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Product Specsheet/SDS
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Part Number: LL-ORELA-A2
The kit contains reagents for the release of N and O-linked glycans from glycoproteins via hydrazinolysis.
Product Specsheet/SDS
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Part Number: LL-HYDRAZ-A2