Monosaccharide analysis is a requirement from ICH Q6B guidelines for lot release characterization of glycoproteins being developed as biopharmaceuticals. This assay provides evidence of the presence or absence of N and O-linked glycans, as well as the relative amounts of each monosaccharide present. When compared to prior lots, this data can provide insight into process variations and provide prof of consistency during the manufacturing process.
The method is reliable and easy to use:
- Release of monosaccharides from the glycoprotein by mild acid hydrolysis
- Fluorescent labeling of released monosaccharides with 2-AA
- Relative quantitative analysis of 2AA-labelled monosaccharides by HPLC or UHPLC
LudgerTag Monosaccharide Kit
This kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards.
View product documentation as PDF
View Certificate of Analysis as PDF
Part Number: LT-MONO-96
LudgerSep uR2 2.1x50mm UHPLC Column
Developed for the analysis of monosaccharides labeled with 2-AA using the LudgerTag Monosaccharide Labeling Kit. The LudgerSep uR2 UHPLC column contains particles with an endcapped octadecylsilane coating optimized for hydrophobic chromatography.
View product documentation as PDF
View Certificate of Analysis as PDF
Dimensions: 2.1mm x 50mm
Part Number: LS-UR2-2.1x50
Ludger Sep R2 4.6x150mm HPLC Column
This reverse phase column resolves glycans based on hydrophobicity.
Applications:
-Analysis of DMB labeled sialic acids
-Analysis of 2-AA labeled monosaccharides
View Certificate of Analysis as PDF
Dimensions: 4.6 x 150mm
Part Number: LS-R2-4.6x150
LudgerSep R BPT Solvent
Monomix Quantitative Standards
A quantitative mix of six monosaccharides:
glucosamine - galactosamine - galactose - mannose - glucose/dextrose - fucose
View product documentation as PDF
Part Number: CM-MONOMIX-10
BioQuant Glycopeptide Standard
The glycopeptide is comprised of an A2G2S2 glycan attached to the asparagine amino acid of a peptide with the sequence Lysine-Valine-Alanine-Asparagine-Lysine-Threonine (KVANKT).
Purity: >90% as determined by UHPLC
Monoisotopic mass: 2865.1763 [M+H]+
Amount: 10 micrograms
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View product documentation as PDF
Part number: BQ-GPEP-A2G2S2-10U
Fetuin 4 x 50µg
Fetuin glycoproteins are purified from fetal calf serum. Fetuin exists in a variety of glycoforms containing bi-, tri-, and tetraantennary N-linked oligosaccharides with variable sialylation, as well as O-linked glycans.
View product documentation/CofA as PDF
4 vials of 50 µg each
Part Number: GCP-FET-50U-X4
Xylose
Xylose standard
High levels of salts may inhibit monosaccharide labeling. Xylose can be used as a internal control to assist in determining whether high salt labeling inhibition is occurring.
100 nanomoles
Part Number: CM-XYL-100