Monosaccharide analysis is a requirement from ICH Q6B guidelines for lot release characterization of glycoproteins being developed as biopharmaceuticals. This assay provides evidence of the presence or absence of N and O-linked glycans, as well as the relative amounts of each monosaccharide present. When compared to prior lots, this data can provide insight into process variations and provide prof of consistency during the manufacturing process.
The method is reliable and easy to use:
- Release of monosaccharides from the glycoprotein by mild acid hydrolysis
- Fluorescent labeling of released monosaccharides with 2-AA
- Relative quantitative analysis of 2AA-labelled monosaccharides by HPLC or UHPLC
The LudgerTag Monosaccharide Analysis Kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards.
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View Certificate of Analysis as PDF
Part Number: LT-MONO-96
A quantitative mix of six monosaccharides:
glucosamine – galactosamine – galactose – mannose – glucose/dextrose – fucose
10 nmols of each monosaccharide per vial
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Download Non-hazardous MSDS
CM-MONOMIX-10 – 1 vial
CM-MONOMIX-10X3 – 3 vials
The LudgerSep uR2 2.1x50mm UHPLC column has been developed for the UHPLC analysis of monosaccharides labeled with 2-AA using the LudgerTag Monosaccharide Analysis Kit.
Dimensions: 2.1mm x 50mm
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View Certificate of Analysis as PDF
Part Number: LS-UR2-2.1×50
The LudgerSep R2 4.6x150mm column has been developed for HPLC analysis of 2-AA labeled monosaccharides labeled with 2-AA using the LudgerTag Monosaccharide Analysis Kit.
View product documents as PDF
View Certificate of Analysis as PDF
Dimensions: 4.6 x 150mm
Part Number: LS-R2-4.6×150