Monosaccharide Analysis

Monosaccharide analysis is a requirement from ICH Q6B guidelines for lot release characterization of glycoproteins being developed as biopharmaceuticals. This assay provides evidence of the presence or absence of N and O-linked glycans, as well as the relative amounts of each monosaccharide present. When compared to prior lots, this data can provide insight into process variations and provide prof of consistency during the manufacturing process.

The method is reliable and easy to use:

  • Release of monosaccharides from the glycoprotein by mild acid hydrolysis
  • Fluorescent labeling of released monosaccharides with 2-AA
  • Relative quantitative analysis of 2AA-labelled monosaccharides by HPLC or UHPLC
Monosaccharide Analysis Kit Image

Monosaccharide Analysis Kit

The LudgerTag Monosaccharide Analysis Kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards.

View product documentation as PDF
View Certificate of Analysis as PDF

Part Number: LT-MONO-96

uR2 2.1x50mm UHPLC Column

uR2 2.1x50mm UHPLC Column

The LudgerSep uR2 2.1x50mm UHPLC column has been developed for the UHPLC analysis of monosaccharides labeled with 2-AA using the LudgerTag Monosaccharide Analysis Kit.

Dimensions: 2.1mm x 50mm

View product documentation as PDF
View Certificate of Analysis as PDF

Part Number: LS-UR2-2.1x50

R2 4.6x150mm HPLC column

R2 4.6x150mm HPLC Column

The LudgerSep R2 4.6x150mm column has been developed for HPLC analysis of 2-AA labeled monosaccharides labeled with 2-AA using the LudgerTag Monosaccharide Analysis Kit.

View product documents as PDF
View Certificate of Analysis as PDF

Dimensions: 4.6 x 150mm

Part Number: LS-R2-4.6x150

LudgerSep R BPT Solvent

LudgerSep R BPT Solvent

The LudgerSep R BPT Solvent was developed for the analysis of monosaccharides labeled with 2-AA using the LudgerTag Monosaccharide Labeling Kit.

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View Certificate of Analysis as PDF

Part Number: LS-R-BPTX10

quantitative monosaccharide standard

Monosaccharide Standard – Quantitative Monomix

A quantitative mix of six monosaccharides:
glucosamine - galactosamine - galactose - mannose - glucose/dextrose - fucose

10 nmols of each monosaccharide per vial

View product documentation as PDF
Download Non-hazardous MSDS

10 nmol each of 6 monosaccharide standards

CM-MONOMIX-10 - 1 vial
CM-MONOMIX-10X3 - 3 vials

$733$1,417
BioQuant Glycopeptide Standard

BioQuant Glycopeptide Standard

This quantitative glycopeptide standard was developed for use during the sialic acid or monosaccharide release and labeling process. This will enable you to check the efficiency of glycan release, labeling, and recovery.

View the BioQuant Presentation

Purity: >90% as determined by UHPLC
Monoisotopic mass: 2865.1763 [M+H]+
Amount: 10 micrograms

View CofA as PDF
Download Non-hazardous MSDS

Part number: BQ-GPEP-A2G2S2-10U

Xylose

Xylose

Xylose can be used as an internal standard when performing monosaccharide analysis. It is a monosaccharide not typically found in mammalian or yeast expression vectors.


Monosaccharide Analysis Guide

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Download Non-hazardous MSDS

100 nanomoles

Part Number: CM-XYL-100