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Monosaccharide Analysis

Monosaccharide analysis is a requirement from ICH Q6B guidelines for lot release characterization of glycoproteins being developed as biopharmaceuticals. This assay provides evidence of the presence or absence of N and O-linked glycans, as well as the relative amounts of each monosaccharide present. When compared to prior lots, this data can provide insight into process variations and provide prof of consistency during the manufacturing process.

The method is reliable and easy to use:

  • Release of monosaccharides from the glycoprotein by mild acid hydrolysis
  • Fluorescent labeling of released monosaccharides with 2-AA
  • Relative quantitative analysis of 2AA-labelled monosaccharides by HPLC or UHPLC
Monosaccharide Analysis Kit Image

Monosaccharide Analysis Kit

The LudgerTag Monosaccharide Analysis Kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards.

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-MONO-96

LudgerSep uR2 2.1x50mm UHPLC Column

Developed for the analysis of monosaccharides labeled with 2-AA using the LudgerTag Monosaccharide Labeling Kit. The LudgerSep uR2 UHPLC column contains particles with an endcapped octadecylsilane coating optimized for hydrophobic chromatography.

View product documentation as PDF

View Certificate of Analysis as PDF

Dimensions: 2.1mm x 50mm

Part Number: LS-UR2-2.1x50

Ludger Sep R2 4.6x150mm HPLC Column

This reverse phase column resolves glycans based on hydrophobicity.

-Analysis of 2-AA labeled monosaccharides

View product documents as PDF

View Certificate of Analysis as PDF

Dimensions: 4.6 x 150mm

Part Number: LS-R2-4.6x150

LudgerSep R BPT Solvent

Use the LS-R-BPTX10 solvent for HPLC analysis with our monosaccharide analysis UHPLC column,the LudgerSep UR2 UHPLC column LS-UR2-2.1x50. View product documentation as PDF View Certificate of Analysis as PDF Part Number: LS-R-BPTX10

Monomix Quantitative Standards

A quantitative mix of six monosaccharides:
glucosamine - galactosamine - galactose - mannose - glucose/dextrose - fucose

10 nmols of each monosaccharide per vial

View product documentation as PDF

CM-MONOMIX-10 - 1 vial
CM-MONOMIX-10X3 - 3 vials

BioQuant Glycopeptide Standard

BioQuant Glycopeptide Standard

The glycopeptide is comprised of an A2G2S2 glycan attached to the asparagine amino acid of a peptide with the sequence Lysine-Valine-Alanine-Asparagine-Lysine-Threonine (KVANKT).

Purity: >90% as determined by UHPLC
Monoisotopic mass: 2865.1763 [M+H]+
Amount: 10 micrograms

Click to view presentation as PDF

View product documentation as PDF

Part number: BQ-GPEP-A2G2S2-10U

Fetuin 4 x 50µg

Fetuin glycoproteins are purified from fetal calf serum. Fetuin exists in a variety of glycoforms containing bi-, tri-, and tetraantennary N-linked oligosaccharides with variable sialylation, as well as O-linked glycans.

View product documentation/CofA as PDF

4 vials of 50 µg each

Part Number: GCP-FET-50U-X4


Xylose, a monosaccharide typically found in plants and insects but not mammalian or yeast expression vectors, can be used as an internal standard (i.e. added to each sample before labelling). This allows compensation for any pipetting/sample preparation errors that may have occurred during sample processing. As well, xylose assists in determining whether high salt labeling inhibition is occurring. High salt may inhibit glycan labeling 100 nanomoles Part Number: CM-XYL-100
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