MALDI Profiling

MALDI-TOF-MS glycan analysis services image

This module provides MALDI-TOF-MS N- and/or O-glycan spectra, and m/z data for mass composition matching of the charged and neutral glycans. The analysis will be performed on a triplicate release of samples (typically 5-100 µg for each release), an equivalent amount (by volume) of sample buffer negative controls, alongside Ludger positive and negative controls and system suitability standards.

This module is suitable for:

  • preliminary glycan assignment
  • quality control – profile comparisons to monitor known structures
  • monitoring batch to batch consistency
  • comparability studies

Workflow for Level 1 MALDI profiling

N-glycans are released from glycoprotein by digestion with PNGAse F or PNGAse A.
O-glycans are released from glycoprotein by hydrazinolysis or Orela reagent.
Released glycans are permethylated, purified and analysed by MALDI-TOF-MS.
Permethylation is the process of derivatizing all the hydroxyl and N-acetyl groups by the addition of a methyl group. Permethylation also methyl esterifies the carboxy function on the sialic acid. Both these derivatizations lead to a stabilization of the sialic acids and to a significant enhancement in the MS analysis.


Final report contains:

    • MALDI-TOF-MS spectra for system suitability standards and Ludger positive controls
    • MALDI-TOF-MS spectra for client samples and buffer negative controls
    • m/z data for mass composition matching of the charged and neutral glycans
    • Relative proportions of detected peaks
    • Summary of findings
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