GlycoAnalytical Services

Ludger logo

Our partners at Ludger Ltd (Oxford, UK) offer a wide range of glycoanalytical services, having over twenty years of experience analyzing glycosylation. They have assisted over half of the world’s pharmaceutical and biotech companies in characterizing their drug’s glycosylation and providing regulatory authorities worldwide with reliable, structured and detailed data. 

Sample types:

  • Biopharmaceuticals: mAbs, glycoprotein hormones (e.g. follicle stimulating hormone (FSH) and erythropoietin (EPO), Fc fusion proteins, vaccines)
  • Cells: mammalian cell lines, bacterial cell components
  • Biological fluids, tissues and others
  • Glycoproteins set in SDS-gel (N-glycans only)
  • COVID-19 patient samples (e.g. plasma, tissues)
  • SARS-CoV-2 infected cell lines

Level 1: Glycoprofiling

monosaccharide analysis image

Quantitative Monosaccharide Analysis

Quantitative data for GlcNAc, GalNAC, Gal, Glc, Fuc and Xyl as nmol/mg protein

sialic acid analysis image

Sialic Acid Analysis

Quantitation of sialic acids (e.g. Neu5Ac, Neu5Gc) as nmol/mg protein

HILIC glycan analysis image

HILIC Profiling

HILIC-UPLC profile for comparability studies.
Glucose Unit (GU) values for preliminary structure assignments
Relative proportions of glycan peak

WAX-HPLC glycan analysis image

WAX Profiling

WAX-HPLC charge profile for comparability studies.
Relative proportions of differently charged glycan peaks

MALDI-TOF-MS glycan analysis services image

MALDI Profiling

MALDI-MS spectra/profile for comparability studies

Level 2: Characterization

exoglycosidase digestion followed by hilic analysis image

Exoglycosidase Digestion Followed by HILIC-HPLC

Structure assignments with monosaccharide type, order and linkage information
Relative proportions of glycan peaks - G0:G1:G2 A1:A2:A3
% Fucosylation : % High mannose : % Galα1-3Gal

wax glycan analysis2 image

WAX-HPLC fractionation followed by exoglycosidase digestion

Identification of N-glycan structures with 0,1,2,3,4 sialic acids
Determine monosaccharide type, linkage, and sequence
Assignments made from exoglycosidase sequencing of each charged fraction



Mass composition sequence data matching if GU, m/z & MS/MS sequencing data
Structure assignments
Relative proportions of glycan peaks - G0:G1:G2 A1:A2:A3
% Fucosylation : % High mannose : % Galα1-3Gal

Level 3: Glycan Site Occupancy

glycan site occupancy image

Site Occupancy

Determine % site occupancy for each glycan site

Site specific glycosylation analysis image

Site Specific Glycosylation

HILIC-UPLC-FLR profiles for the N-glycans at each site
Complete sequence information for each occupied site

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QA-Bio - a distributor of Ludger products since 2002