Our partners at Ludger Ltd (Oxford, UK) offer a wide range of glycoanalytical services, having over twenty years of experience analyzing glycosylation. They have assisted over half of the world’s pharmaceutical and biotech companies in characterizing their drug’s glycosylation and providing regulatory authorities worldwide with reliable, structured and detailed data.
Sample types:
- Biopharmaceuticals: mAbs, glycoprotein hormones (e.g. follicle stimulating hormone (FSH) and erythropoietin (EPO), Fc fusion proteins, vaccines)
- Cells: mammalian cell lines, bacterial cell components
- Biological fluids, tissues and others
- Glycoproteins set in SDS-gel (N-glycans only)
- COVID-19 patient samples (e.g. plasma, tissues)
- SARS-CoV-2 infected cell lines
Level 1: Glycoprofiling
Quantitative Monosaccharide Analysis
Quantitative data for GlcNAc, GalNAC, Gal, Glc, Fuc and Xyl as nmol/mg protein
Sialic Acid Analysis
Quantitation of sialic acids (e.g. Neu5Ac, Neu5Gc) as nmol/mg protein
HILIC Profiling
HILIC-UPLC profile for comparability studies.
Glucose Unit (GU) values for preliminary structure assignments
Relative proportions of glycan peak
WAX Profiling
WAX-HPLC charge profile for comparability studies.
Relative proportions of differently charged glycan peaks
MALDI Profiling
MALDI-MS spectra/profile for comparability studies
Level 2: Characterization
Exoglycosidase Digestion Followed by HILIC-HPLC
Structure assignments with monosaccharide type, order and linkage information
Relative proportions of glycan peaks - G0:G1:G2 A1:A2:A3
% Fucosylation : % High mannose : % Galα1-3Gal
WAX-HPLC fractionation followed by exoglycosidase digestion
Identification of N-glycan structures with 0,1,2,3,4 sialic acids
Determine monosaccharide type, linkage, and sequence
Assignments made from exoglycosidase sequencing of each charged fraction
HILIC-UPLC-FLR-ESI-MS/MS
Mass composition sequence data matching if GU, m/z & MS/MS sequencing data
Structure assignments
Relative proportions of glycan peaks - G0:G1:G2 A1:A2:A3
% Fucosylation : % High mannose : % Galα1-3Gal
Level 3: Glycan Site Occupancy
Site Occupancy
Determine % site occupancy for each glycan site
Site Specific Glycosylation
HILIC-UPLC-FLR profiles for the N-glycans at each site
Complete sequence information for each occupied site
