Acetic Acid – Promotes acid catalyzed ring opening of the glycan reducing terminus prior to the formation of an imine intermediate between the sugars and the unprotonated form of the dye
Fluorescent Dye – The comprehensive range of LudgerTag dyes include 2-AB, 2-AA, Procainamide, DMB, and APTS to cover different types of glycoanalyses by HPLC, CE, and MS.
DMSO – An anhdrous solvent used to dissolve all the components of the reductive amination labeling reaction.
Sodium Cyanoborohydride or Non-Toxic 2PB – This reductant stabilizes the imine produced by the reaction of the dye and glycans to produce stable fluorescently labeled glycan
Procainamide labeling permits glycan identification by either mass spectrometry or UHPLC. Because of its improved ionisation efficiency compared to 2-AB labelling, it can permit identification of minor glycans (1% relative peak area) by ESI-MS.
Part Number Reductant
LT-KPROC-24 Sodium Cyanoborohydride
LT-KPROC-VP24 2-Picoline Borane
This kit is used for the permethylation of glycans. Permethylation stabilizes sialic acids for MALDI mass spectrometry analysis, and aids linkage analysis studies (MALDI-MS, ESI-MS).
- Improves and enhances ionization efficiency of glycans on mass spec
- Increased glycan hydrophobicity enables LC-MS analysis
- Relative and absolute quantitation can be performed
- Stabilizes the sialic acid moieties
- Easier determination of branching and glycosidic linkage positions
- Enables detection of both neutral and acidic glycans in positive ion mode
Part Number Reductant
LT-PERMET-VP24 2-Picoline Borane
The LudgerTag Monosaccharide Analysis Kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards.
Part Number: LT-MONO-96
The LudgerTag DMB Sialic Acid Labeling Kit has been developed for the qualitative analysis of sialic acids
The kit contains reagents for the release of sialic acids from glycoproteins. Released sialic acids are conjugated with DMB dye by an amination-cyclisation reaction.
The kit contains reagents and materials for up to 22 samples including the sialic acid reference panel, and the N-acetyl neuraminic acid and N-glycolyl neuraminic acid standards.
Part Number: LT-KDMB-A1
The LudgerTag V-tag labeling kit is designed for the fluorophore labeling of enzymatically digested peptides and glycopeptides followed by enrichment of the labeled glycopeptides. The V-tag labeled glycopeptides are analyzed using MALDI-MS and HPLC/UHPLC
Part Number: LT-VTAG-24