This kit includes the enzymes and buffers required to deglycosylate glycoproteins, removing all N-linked oligosaccharides and many O-linked sugars.
The enzymes are packaged separately in individual 20 microlitre vials. This gives researchers the flexibility to characterize the glycans attached to their glycoprotein more fully than with our mixture of enzymes, KE-DGMX.
Part Number: KE-DG01
This kit developed for protein deglycosylation includes our DeGlycoMx, a premixed cocktail of the enzymes required to remove all N-linked oligosaccharides and most O-linked sugars from 0.5 mg of glycoprotein, via 10 reactions of up to 50 micrograms of protein per reaction.
The kit is easy-to-use and effective: add 2 µl of the DeGlycoMx enzyme to your denatured sample and the included buffer and incubate for 3 hours at 37˚C.
KE-DGMX - 20 microliters
KE-DGMX-100 - 100 microliters
The Endo F Multi-Kit will deglycosylate N-linked glycans in both native and denatured conditions. Each enzyme has a distinct specificity for N-linked glycan release. One can choose to use the three enzymes in combination to completely remove all N-linked glycans present on a glycoprotein or peptide, or to use each enzyme independently and thereby determine the type of N-glycans present.
Part Number: KE-EFX3