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Deglycosylation Kits

Deglycosylate glycoproteins image

Enzymatic CarboRelease Kit

This kit includes the enzymes and buffers required to deglycosylate glycoproteins, removing all N-linked oligosaccharides and many O-linked sugars.

The enzymes are packaged separately in individual 20 microlitre vials. This gives researchers the flexibility to characterize the glycans attached to their glycoprotein more fully than with our mixture of enzymes, KE-DGMX.

View product documentation:
Specsheet   CofA  MSDS

Part Number: KE-DG01

protein deglycosylation image

Enzymatic DeGlycoMx Kit

This kit developed for protein deglycosylation includes our DeGlycoMx, a premixed cocktail of the enzymes required to remove all N-linked oligosaccharides and most O-linked sugars from 0.5 mg of glycoprotein, via 10 reactions of up to 50 micrograms of protein per reaction.

The kit is easy-to-use and effective: add 2 µl of the DeGlycoMx enzyme to your denatured sample and the included buffer and incubate for 3 hours at 37˚C.

View product documentation:
Specsheet   CofA   MSDS

Part Number:
KE-DGMX - 20 microliters
KE-DGMX-100 - 100 microliters

Endo F Multi-kit image

Endo F Multi-Kit

The Endo F Multi-Kit will deglycosylate N-linked glycans in both native and denatured conditions. Each enzyme has a distinct specificity for N-linked glycan release. One can choose to use the three enzymes in combination to completely remove all N-linked glycans present on a glycoprotein or peptide, or to use each enzyme independently and thereby determine the type of N-glycans present.

View product documentation:
Specsheet   CofA  MSDS

Part Number: KE-EFX3

ORELA O-linked deglycosylation image

Orela O-Linked Glycan Release Kit

The Ludger Liberate™ Orela Kit has been developed for O-linked deglycosylation of glycoproteins. Released glycans have free reducing terminii to allow fluorescent tagging by reductive amination.View product documentation as PDFPart Number: LL-ORELA-A2
Hydrazinolysis image

Hydrazinolysis Kit

The kit contains reagents for the release of N and O-linked glycans from glycoproteins via hydrazinolysis.

View product documentation as PDF

Part Number: LL-HYDRAZ-A2

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